Recombinant antibodies
Recombinant research antibodies provide consistent, animal-free, and highly specific binding reagents for a wide range of applications. Using advanced recombinant antibody discovery platforms, such as phage display and single B-cell antibody technology, our high-quality recombinant monoclonal antibodies are tailored for research use. In phage display, antibody fragments like single-chain variable fragment (scFv) are displayed on bacteriophages, allowing rapid selection from vast immune or naïve libraries, without the variability of animal immunisation. Single B-cell technology isolates and sequences antibody genes from individual B cells, preserving native heavy and light chain pairing for optimal specificity.
Our recombinant research antibodies are produced using optimised expression systems, including mammalian cells like CHO and HEK for full-length antibodies with correct folding and post-translational modifications. Following production, each batch undergoes stringent purification and characterisation to ensure reproducible performance in techniques, including western blot, immunofluorescence, flow cytometry and ELISA.
Featured recombinant antibodies
Recombinant Antibodies for Cell Biology
Recombinant antibodies are essential tools in cell biology, particularly for observing and quantifying dynamic cellular processes in real time. The stability, specificity and consistency across different batches make recombinant antibodies ideal for live-cell imaging and tracking to study activities like protein trafficking, cytoskeletal rearrangements, cell division and receptor-ligand interactions. Cytoskeleton, organelles, and stress pathways for imaging and WB.
| Target | Application | Notes | Browse |
|---|---|---|---|
| β-Actin (ACTB) | WB loading control | ~42 kDa cytoskeletal marker | ACTB recombinant antibodies |
| α/β-Tubulin | WB/IF cytoskeleton | Microtubule network | Tubulin recombinant antibodies |
| Lamin A/C (LMNA/C) | Nuclear envelope | Organelle identity marker | Lamin recombinant antibodies |
| HSP70 (HSPA1A) | Stress response | Chaperone; heat shock marker | HSP70 recombinant antibodies |
Recombinant Antibodies for Immunology
Uncover the dynamic dance between immune cells by visualising crucial interactions like the formation of the immunological synapse between T-cells and B-cells, providing clarity and precision at the cellular level. Additionally, these antibodies enable the accurate tracking of immune cell migration towards sites of inflammation, offering vital insights into how the immune system responds to infection and disease.
Achieve reliable and reproducible results every time, thanks to their superior specificity, high sensitivity, and batch-to-batch consistency, making them perfect for a wide range of applications from flow cytometry to immunofluorescence.
| Target | Use | Notes | Browse |
|---|---|---|---|
| CD3 / CD4 / CD8 | T-cell phenotyping (FC/IF) | Pan-T and subset markers | T-cell marker recombinant antibodies |
| CCL3 (MIP-1α) | Chemokine profiling | Inflammation & migration | CCL3 recombinant antibodies |
| PD-1 / PD-L1 | Checkpoint studies | Exhaustion & immunotherapy | PD-1/PD-L1 recombinant antibodies |
Recombinant Antibodies for Neuroscience
Engineered to be the ideal tool for visualising key events in immunology, offering exceptional specificity and sensitivity. Recombinant antibodies allow researchers to uncover the dynamic dance between immune cells by visualising crucial interactions like the formation of the immunological synapse between T-cells and B-cells, providing clarity and precision at the cellular level. Additionally, these antibodies enable the accurate tracking of immune cell migration towards sites of inflammation, offering vital insights into how the immune system responds to infection and disease.
With our recombinant antibodies, you can achieve reliable and reproducible results every time, thanks to their superior specificity, high sensitivity, and batch-to-batch consistency, making them perfect for a wide range of applications from flow cytometry to immunofluorescence.
| Target | Use | Notes | Browse |
|---|---|---|---|
| NeuN (RBFOX3) | Neuron ID (IHC/IF) | Loss indicates neuronal damage | NeuN recombinant antibodies |
| GFAP | Astrocyte marker | Gliosis & neuroinflammation | GFAP recombinant antibodies |
| Iba1 (AIF1) | Microglia activation | Immune response in CNS | Iba1 recombinant antibodies |
| Synaptophysin | Synapse density | Presynaptic vesicles | SYP recombinant antibodies |
| Amyloid-β (Aβ40) | AD models | Plaques/aggregates | Aβ40 recombinant antibodies |
Recombinant Antibodies for Oncology
Understanding the mechanisms that drive cancer progression is essential for developing effective therapies. Our recombinant research antibodies provide reliable and specific tools for studying key processes in oncology, including the invasion and metastasis of cancer cells, the effects of therapeutic antibodies on tumour growth and signaling pathways, and the molecular mechanisms of cell death such as apoptosis and autophagy. Designed for consistency and reproducibility, recombinant antibodies enable tracking of dynamic changes in tumour biology, investigate drug responses, and uncover new therapeutic targets.
| Target | Axis/Use | Notes | Browse |
|---|---|---|---|
| BCL-2 | Apoptosis | Pro-/anti-apoptotic balance | BCL-2 recombinant antibodies |
| BAX | Apoptosis | Pro-/anti-apoptotic balance | BAX recombinant antibodies |
| Caspase-1/7 | Apoptosis | Pro-/anti-apoptotic balance | CASP1/7 recombinant antibodies |
| AKT | AKT | Total + phospho readouts | AKT recombinant antibodies |
| p-AKT (S473) | PI3K | Total + phospho readouts | pAKT recombinant antibodies |
| MEK1 | MAPK | Stress & growth signals | MAPK recombinant antibodies |
| p38 (p-T180/Y182) | MAPK | Stress & growth signals | MAPK recombinant antibodies |
| EGFR | Biomarkers | Surface & secreted markers | EGFR recombinant antibodies |
| EpCAM | Biomarkers | Surface & secreted markers | EpCAM recombinant antibodies |
| MUC1 | Biomarkers | Surface & secreted markers | MUC1 recombinant antibodies |
| CA9 | Biomarkers | Surface & secreted markers | CA9 recombinant antibodies |
| p53 (TP53) | Tumour suppressor | DNA damage response | p53 recombinant antibodies |
Recombinant Antibodies for DNA Repair & Genomic Stability
Defects in DNA repair pathways are a significant driver of genomic instability and cancer. Recombinant antibodies against key mismatch repair proteins provide reliable tools for research. These antibodies enable researchers to accurately assess the status of these crucial DNA repair proteins, providing critical insights for advancing research.
| Target | Pathway | Notes | Browse |
|---|---|---|---|
| MLH1 | MMR | Marker for MSI testing workflows | MLH1 recombinant antibodies |
| PMS2 | MMR | Pairs with MLH1 for panels | PMS2 recombinant antibodies |
Recombinant Antibody Formats
Recombinant antibodies are engineered proteins produced from defined DNA sequences, offering consistent quality, high specificity, and reproducibility for research applications. Unlike traditional hybridoma-derived antibodies, recombinant antibodies are fully characterized and free from lot-to-lot variability, making them ideal for sensitive assays and advanced imaging studies. Pick the format that matches your assay and detection system.
| Format | Best for | Notes | Browse |
|---|---|---|---|
| Full-length IgG | WB, IF/IHC, FC, ELISA, IP | Native effector & bivalency | Recombinant IgG |
| scFv | Imaging, capture, HCI | Small size; no Fc interactions | scFv recombinant antibodies |
| Fab / fragments | Reduced steric hindrance | Improved tissue penetration | Fab fragments |
| Tagged/Conjugated | Direct IF/FC/WB | AF dyes, PE/APC, HRP/AP | Conjugated |
Advantages
Recombinant antibodies have improved yield, quality and homogeneity by eliminating process variables such as hybridoma instability and supply inconsistencies. Our recombinant antibodies offer researchers a dependable solution for accurate, reproducible results in discovery and validation workflows. The benefits of recombinant antibodies are clear in their consistent performance and animal-free production. We use advanced recombinant antibody technology to create high-quality products.
| Advantage | Details | Notes |
|---|---|---|
| Reproducibility | Fixed sequence; controlled expression | Eliminates hybridoma drift |
| Specificity | Library selection or native B-cell pairing | Reduced off-target binding |
| Engineering | Easy Fc, scFv, Fab, tag/dye fusions | Custom formats for assays |
| Ethics & supply | Animal-free; scalable manufacturing | Consistent availability |
High-Content Imaging (HCI)
Large datasets demand sequence-defined probes for reproducibility, multiplexing, and high specificity, which are key reasons HCI labs favour recombinant antibodies. This technique is widely used in various research fields, particularly in drug discovery and basic research that require a quantitative and high-throughput approach.
HCI utilizes automated microscopes and image analysis software to simultaneously measure multiple parameters within individual cells, such as protein localization, cell shape, nuclear size, and cytoskeletal integrity. This is done across thousands of cells in a single experiment.
| Need | Why recombinant helps | Notes |
|---|---|---|
| Reproducibility | Fixed sequence; identical lots | Reduces assay-to-assay noise |
| Multiplexing | Custom dye/tagged variants | Panel building without cross-reactivity |
| Specificity | Rigorous selection/validation | Cleaner signals for quantitation |
Validation & QC
Each recombinant lot ships with defined specs and is amenable to stringent functional validation. Review affinity, specificity, epitope details, conjugation/format, and storage guidance on the COA/datasheet.
| Attribute | Typical info | Why it matters |
|---|---|---|
| Affinity (KD) | Low nM–pM (target-dependent) | Sensitivity & retention |
| Specificity | Competition & cross-reactivity panels | Minimises off-target signal |
| Epitope | Linear vs conformational | Guides fixation/denaturation |
| Conjugate/format | AF/PE/APC/HRP/AP | One-step detection vs secondary |
| Storage | Lyophilised or −20 to −80 °C | Preserves activity |
Recombinant vs Hybridoma (Quick Compare)
| Feature | Recombinant | Hybridoma-derived |
|---|---|---|
| Lot consistency | High (fixed sequence) | Variable (clone drift) |
| Engineering | Flexible (Fc/scFv/Fab/tags) | Limited |
| Animal use | Animal-free production | Animal immunisation |
| Scale & supply | Scalable expression | Hybridoma stability dependent |
FAQs
| Question | Answer |
|---|---|
| How are recombinant antibodies different from hybridoma products? | They are produced from a defined sequence in an expression system, giving consistent lots and enabling custom formats (Fc, scFv, tags) without hybridoma drift. |
| Do recombinant antibodies work in fixed tissues? | Yes they can. We recommend checking the epitope and recommended fixation. Conformational epitopes may require mild retrieval or optimized permeabilisation. |
| Can I get the same clone in multiple conjugates? | We can provide a range of conjugation options for recombinant antibodies bought in bulk >1mL. Please enquire for further details. |
Need help choosing a recombinant antibody?
Tell us your target, application (WB/IF/FC/ELISA/IP), species, and any conjugation needs. We’ll recommend clones and share lot-specific QC.