Rat VCAM1 (Vascular Cell Adhesion Molecule 1) Sandwich ELISA Kit (STJE0012980)
SPECIFICATIONS
General Information
| Short Description | This VCAM1 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 5.2pg/mL |
| Detection Limit | 12.5-800pg/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | Vcam1 |
| Gene ID | 25361 |
| Uniprot ID | VCAM1_RAT |
| Sample Type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in aortic endothelial cells, with low expression in the descending thoracic aorta and the outer curvature of the aortic arch, where pulsatory shear stress exists, and high in the inner curvature of the aortic arch, where oscillatory shear stress prevails (at protein level). Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. |
| Post Translational Modifications | Cleaved by the metalloproteinase ADAM17 to generate the soluble form. Sialoglycoprotein. Ubiquitinated by TRIM65 via 'Lys-48'-linked ubiquitination.leading to proteasomal degradation. |
| Function | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation. Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins. During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration. Also serves as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation. |
| Protein Name | Vascular Cell Adhesion Protein 1V-Cam 1Vcam-1Cd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 |
| Database Links | Reactome: R-RNO-198933Reactome: -RNO-216083 |
| Cellular Localisation | Vascular Cell Adhesion Protein 1: Cell MembraneSingle-Pass Type I Membrane ProteinSoluble Vascular Cell Adhesion Molecule-1: Secreted |
| Alternative ELISA Names | Vascular Cell Adhesion Protein 1 ELISA kitV-Cam 1 ELISA kitVcam-1 ELISA kitCd Antigen Cd106 Cleaved Into - Soluble Vascular Cell Adhesion Molecule-1 ELISA kitVcam1 ELISA kitVcam-1 ELISA kit |
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Information sourced from Uniprot.org