Rat TM (Thrombomodulin) Sandwich ELISA Kit (STJE0006155)
SPECIFICATIONS
General Information
| Short Description | This TM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 56.25pg/mL |
| Detection Limit | 93.75~6000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Specificity | This kit recognizes Rat TM in samples. No significant cross-reactivity or interference between Rat TM and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Background | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat TM. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TM and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TM, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat TM. You can calculate the concentration of Rat TM in the samples by comparing the OD of the samples to the standard curve. |
Information sourced from Uniprot.org