Protein Name | Serum Amyloid P-ComponentSap |
Cellular Localisation | Secreted |
Alternative ELISA Names | Serum Amyloid P-Component ELISA kitSap ELISA kitApcs ELISA kitPtx2 ELISA kitSap ELISA kit |
Specificity | This kit recognizes Rat SAP in samples. No significant cross-reactivity or interference between Rat SAP and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-98 | 91 |
EDTA plasma (n=8) | 90-101 | 95 |
Cell culture media (n=8) | 89-103 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 105.20 | 218.76 | 702.05 | 98.45 | 239.37 | 760.25 |
Standard deviation | 6.58 | 12.93 | 30.12 | 6.10 | 13.19 | 27.22 |
CV (%) | 6.25 | 5.91 | 4.29 | 6.20 | 5.51 | 3.58 |