• Rat PGF (Placental Growth Factor) CLIA Kit (STJC0001836)

Rat PGF (Placental Growth Factor) CLIA Kit (STJC0001836)

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STJC0001836

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 1.88pg/mL
Detection Limit: 3.13~200pg/mL
Short Description: This rat PGF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Pgf
Gene ID: 94203
Uniprot ID: PLGF_RAT
Specificity: This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Also promotes cell tumor growth.
Protein Name Placenta Growth Factor
Plgf
Database Links Reactome: R-RNO-194313
Reactome: -RNO-195399
Cellular Localisation Secreted
Alternative CLIA Names Placenta Growth Factor CLIA kit
Plgf CLIA kit
Pgf CLIA kit
Plgf CLIA kit
Specificity This kit recognizes Rat PGF in samples. No significant cross-reactivity or interference between Rat PGF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-111 104
EDTA plasma(n=8) 85-98 91
Cell culture media(n=8) 97-111 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 9.38 23.95 97.83 8.91 22.41 100.36
Standard deviation 0.97 2.16 7.87 0.87 2.20 8.68
CV (%) 10.34 9.02 8.04 9.76 9.82 8.65
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance