Post Translational Modifications | Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation. |
Function | Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8. |
Protein Name | Nitric Oxide Synthase - InducibleInducible No SynthaseInducible NosInosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2 |
Database Links | Reactome: R-RNO-1222556Reactome: -RNO-392154Reactome: -RNO-9033241 |
Cellular Localisation | CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization |
Alternative ELISA Names | Nitric Oxide Synthase - Inducible ELISA kitInducible No Synthase ELISA kitInducible Nos ELISA kitInos ELISA kitNos Type Ii ELISA kitPeptidyl-Cysteine S-Nitrosylase Nos2 ELISA kitNos2 ELISA kit |
Specificity | This kit recognizes Rat NOS2/iNOS in samples. No significant cross-reactivity or interference between Rat NOS2/iNOS and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 86-97 | 92 |
EDTA plasma (n=8) | 89-101 | 96 |
Cell culture media (n=8) | 89-102 | 94 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (ng/mL) | 1.00 | 2.70 | 8.10 | 1.10 | 2.70 | 7.70 |
Standard deviation | 0.10 | 0.10 | 0.40 | 0.10 | 0.10 | 0.40 |
CV (%) | 10.00 | 3.70 | 4.94 | 9.09 | 3.70 | 5.19 |