| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of 20mM Tris, 500mM NaCl, pH 7.0 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
< 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: |
Measured by its ability to bind Iron (III) dihydroxybenzoic acid [Fe (DHBA) 3]. The binding of Fe (DHBA) 3 results in the quenching of Trp fluorescence in Lipocalin2. It binds >51330 Mu M of Fe (DHBA) 3. |
| Gene Symbol: |
Lcn2 |
| Gene ID: |
170496 |
| Uniprot ID: |
NGAL_RAT |
| Immunogen: |
Recombinant Rat Lipocalin-2/NGAL/LCN2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln21-Asn198) of rat Lipocalin-2/NGAL (Accession #NP_570097.1.) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: |
QDSTQNLIPAPPLISVPLQP GFWTERFQGRWFVVGLAANA VQKERQSRFTMYSTIYELQE DNSYNVTSILVRGQGCRYWI RTFVPSSRPGQFTLGNIHSY PQIQSYDVQVADTDYDQFAM VFFQKTSENKQYFKVTLYGR TKGLSDELKERFVSFAKSLG LKDNNIVFSVPTDQCIDN |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated LipocalinNgalAlpha-2-Microglobulin-Related ProteinAlpha-2u Globulin-Related ProteinLipocalin 24p3Lipocalin-2Siderocalin Lcn2P25 |
| Database Links | Reactome: R-RNO-6798695Reactome: -RNO-6799990Reactome: -RNO-917937 |
| Cellular Localisation | SecretedCytoplasmic Granule LumenCytoplasmic Vesicle LumenUpon Binding To The Slc22a17 (24p3r) ReceptorIt Is InternalizedReleases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative Protein Names | Neutrophil Gelatinase-Associated Lipocalin proteinNgal proteinAlpha-2-Microglobulin-Related Protein proteinAlpha-2u Globulin-Related Protein proteinLipocalin 24p3 proteinLipocalin-2 proteinSiderocalin Lcn2 proteinP25 proteinLcn2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
