Rat Ldha (L-lactate dehydrogenase A chain) Sandwich ELISA Kit (STJE0006487)
SPECIFICATIONS
General Information
| Short Description | This Ldha Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 0.19ng/mL |
| Detection Limit | 0.31~20ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | Ldha |
| Gene ID | 24533 |
| Uniprot ID | LDHA_RAT |
| Specificity | This kit recognizes Rat LDHA in samples. No significant cross-reactivity or interference between Rat LDHA and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+). |
| Protein Name | L-Lactate Dehydrogenase A ChainLdh-ALdh Muscle SubunitLdh-M |
| Database Links | Reactome: R-RNO-70268Reactome: -RNO-9861718 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | L-Lactate Dehydrogenase A Chain ELISA kitLdh-A ELISA kitLdh Muscle Subunit ELISA kitLdh-M ELISA kitLdha ELISA kitLdh-1 ELISA kitLdh1 ELISA kit |
| Specificity | This kit recognizes Rat LDHA in samples. No significant cross-reactivity or interference between Rat LDHA and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 85-99 | 92 |
| EDTA plasma (n=8) | 95-107 | 101 |
| Cell culture media (n=8) | 89-105 | 96 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.99 | 2.07 | 9.25 | 0.92 | 2.07 | 8.77 |
| Standard deviation | 0.07 | 0.10 | 0.40 | 0.06 | 0.10 | 0.31 |
| CV (%) | 7.07 | 4.83 | 4.32 | 6.52 | 4.83 | 3.53 |