| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <0.01 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 0.65-2.6 Mu g/mL, corresponding to a specific activity of 3.85×102~1.54×103 units/mg. |
| Gene Symbol: | Il4 |
| Gene ID: | 287287 |
| Uniprot ID: | IL4_RAT |
| Immunogen Region: | Cys25-Ser147 |
| Immunogen: | Recombinant Rat IL-4 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Cys25-Ser147) of Rat IL-4 (Accession #NP_958427.1) fused with a His at the C-terminus. |
| Immunogen Sequence: | VRERGPQRVAAHITGTRGRS NTLSSPNSKNEKALGRKINS WESSRSGHSFLSNLHLRNGE LVIHEKGFYYIYSQTYFRFQ EEIKENTKNDKQMVQYIYKY TSYPDPILLMKSARNSCWSK DAEYGLYSIYQGGIFELKEN DRIFVSVTNEHLIDMDHEAS FFGAFLVG |
| Function | Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. |
| Protein Name | Interleukin-4Il-4B-Cell Igg Differentiation FactorB-Cell Growth Factor 1B-Cell Stimulatory Factor 1Bsf-1Lymphocyte Stimulatory Factor 1 |
| Database Links | Reactome: R-RNO-6785807 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Interleukin-4 proteinIl-4 proteinB-Cell Igg Differentiation Factor proteinB-Cell Growth Factor 1 proteinB-Cell Stimulatory Factor 1 proteinBsf-1 proteinLymphocyte Stimulatory Factor 1 proteinIl4 proteinIl-4 protein |
Information sourced from Uniprot.org