| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | <1 EU/Mu g |
| Immunoreactivity: | Recombinant Rat IL-1 beta Protein stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.68-2.72 ng/mL, corresponding to a specific activity of 3.68×105~1.47×106 units/mg |
| Immunogen Region: | Val117-Ser268 |
| Immunogen: | Recombinant Rat IL-1 beta Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val117-Ser268) of rat IL-1 beta (Accession #NP_113700.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Sequence: | VPIRQLHCRLRDEQQKCLVL SDPCELKALHLNGQNISQQV VFSMSFVQGETSNDKIPVAL GLKGKNLYLSCVMKDGTPTL QLESVDPKQYPKKKMEKRFV FNKIEVKTKVEFESAQFPNW YISTSQAEHRPVFLGNSNGR DIVDFTMEPVSS |