Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Antibody Pair (STJAP00089)
General Information
| Short Description | This ICAM-1/CD54 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Rat |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | Icam1 |
| Gene ID | 25464 |
| Uniprot ID | ICAM1_RAT |
| Immunogen | Capture immunogen: Recombinant Rat ICAM-1/CD54 protein.Detection Immunogen: Recombinant Rat ICAM-1/CD54 protein. |
Additional Info
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. |
| Protein Name | Intercellular Adhesion Molecule 1Icam-1Cd Antigen Cd54 |
| Database Links | Reactome: R-RNO-198933Reactome: -RNO-216083 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Intercellular Adhesion Molecule 1 antibody pairIcam-1 antibody pairCd Antigen Cd54 antibody pairIcam1 antibody pairIcam-1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1) Antibody Pair (STJAP00089)
This antibody pair detects rat ICAM-1/CD54 (Intercellular Adhesion Molecule 1), corresponding to UniProt entry Q00238. Reactivity has been confirmed for rat only.
The capture antibody is an unconjugated mouse monoclonal antibody supplied at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4. The detection antibody is a biotin-conjugated goat polyclonal antibody supplied at the same concentration and in the same buffer formulation.
This antibody pair has been validated for use in ELISA. No other assay formats are specified in the product documentation.
Both the capture and detection antibodies were raised against recombinant rat ICAM-1/CD54 protein. The capture antibody was purified by Protein A or G, while the detection antibody was purified by antigen affinity.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.