Rat Hepatocyte growth factor/HGF protein (Recombinant) (No-Tag) (STJP002970)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Rat Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 33-Leu728) of rat HGF (Accession #NP_058713.1) fused with and a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Rat Hepatocyte growth factor/HGF-No-Tag protein was developed in e.coli using the region Gln 33-Leu728. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using Mv.1.lu cells. The ED50 for this effect is 1.6-6.2 ng/mL, corresponding to a specific activity of 1.6×105~6.3×105 units/mg. |
| Determination Method | <0.001 EU/Mu g |
Target Information
| Gene Symbol | Hgf |
| Gene ID | 24446 |
| Uniprot ID | HGF_RAT |
| Immunogen | Recombinant Rat Hepatocyte growth factor/HGF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln 33-Leu728) of rat HGF (Accession #NP_058713.1) fused with and a 6×His tag at the C-terminus. |
| Immunogen Region | Gln 33-Leu728 |
| Immunogen Sequence | MQGPRTRPRKPIRCGEVLFA VGGWCSGDAISSVERYDPQT NEWRMVASMSKRRCGVGVSV LDDLLYAVGGHDGSSYLNSV ERYDPKTNQWSSDVAPTSTC RTSVGVAVLGGFLYAVGGQD GVSCLNIVERYDPKENKWTR VASMSTRRLGVAVAVLGGFL YAVGGSDGTSPLNTVERYNP QENRWHTIAPMGTRRKHLGC AVYQDMIYAVGGRDDTTELS SAERYNPRTNQWSPVVAMT |
Additional Info
| Post Translational Modifications | The single-chain precursor undergoes proteolytic processing by TMPRSS13 resulting in an active two-chain form. The single-chain precursor undergoes proteolytic processing by HGFAC resulting in an active two-chain form. |
| Function | Potent mitogen for mature parenchymal hepatocyte cells, seems to be a hepatotrophic factor, and acts as a growth factor for a broad spectrum of tissues and cell types. Activating ligand for the receptor tyrosine kinase MET by binding to it and promoting its dimerization. Activates MAPK signaling following TMPRSS13 cleavage and activation. |
| Protein Name | Hepatocyte Growth FactorHepatopoietin-AScatter FactorSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain |
| Database Links | Reactome: R-RNO-114608Reactome: -RNO-1257604Reactome: -RNO-5673001Reactome: -RNO-6806942Reactome: -RNO-6807004Reactome: -RNO-6811558Reactome: -RNO-8851805Reactome: -RNO-8851907Reactome: -RNO-8865999Reactome: -RNO-8874081Reactome: -RNO-8875513Reactome: -RNO-8875555Reactome: -RNO-8875656Reactome: -RNO-8875791Reactome: -RNO-9734091 |
| Cellular Localisation | |
| Alternative Protein Names | Hepatocyte Growth Factor proteinHepatopoietin-A proteinScatter Factor proteinSf Cleaved Into - Hepatocyte Growth Factor Alpha Chain - Hepatocyte Growth Factor Beta Chain proteinHgf protein |
Information sourced from Uniprot.org