| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.19mIU/mL |
| Detection Limit: |
0.31~20mIU/mL |
| Short Description: |
This rat DRD2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Drd2 |
| Gene ID: |
24318 |
| Uniprot ID: |
DRD2_RAT |
| Specificity: |
This kit recognizes Rat DRD2 in samples. No significant cross-reactivity or interference between Rat DRD2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Isoform Long: Expressed in the anterior lobe of the pituitary gland. Expressed ventral tegmental area of the midbrain and the pars compacta of the substantia nigra. Expressed seven times more than isoform short in the caudate nucleus. Isoform Short: Expressed in the anterior lobe of the pituitary gland. Expressed in the caudate nucleus. Not expressed in the wider brain. |
| Post Translational Modifications | Palmitoylated. Palmitoylation which is required for proper localization to the plasma membrane and stability of the receptor could be carried on by ZDHHC4, ZDHHC3 and ZDHHC8. |
| Function | Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Positively regulates postnatal regression of retinal hyaloid vessels via suppression of VEGFR2/KDR activity, downstream of OPN5. |
| Protein Name | D(2 Dopamine ReceptorDopamine D2 Receptor |
| Database Links | Reactome: R-RNO-390651 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinGolgi Apparatus Membrane |
| Alternative CLIA Names | D(2 Dopamine Receptor CLIA kitDopamine D2 Receptor CLIA kitDrd2 CLIA kit |
| Specificity | This kit recognizes Rat DRD2 in samples. No significant cross-reactivity or interference between Rat DRD2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 87-100 | 93 |
| EDTA plasma(n=8) | 84-98 | 90 |
| Cell culture media(n=8) | 96-109 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (mIU/mL) | 1.05 | 2.58 | 7.97 | 0.98 | 2.71 | 7.81 |
| Standard deviation | 0.10 | 0.28 | 0.55 | 0.12 | 0.20 | 0.79 |
| CV (%) | 9.52 | 10.85 | 6.90 | 12.24 | 7.38 | 10.12 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
