| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
1.88ng/mL |
| Detection Limit: |
3.13~200ng/mL |
| Short Description: |
This rat DRD1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Drd1Uniprot ID=DRD1_RAT" |
| Specificity: |
This kit recognizes Rat DRD1 in samples. No significant cross-reactivity or interference between Rat DRD1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Brain, in the striatum, the nucleus accumbens, and the olfactory tubercle. |
| Post Translational Modifications | N-glycosylated. |
| Function | Dopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase. |
| Protein Name | D(1a Dopamine ReceptorDopamine D1 Receptor |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinEndoplasmic Reticulum MembraneCell ProjectionDendriteDendritic SpineTransport From The Endoplasmic Reticulum To The Cell Surface Is Regulated By Interaction With Dnajc14 |
| Alternative CLIA Names | D(1a Dopamine Receptor CLIA kitDopamine D1 Receptor CLIA kitDrd1 CLIA kitDrd1a CLIA kit |
| Specificity | This kit recognizes Rat DRD1 in samples. No significant cross-reactivity or interference between Rat DRD1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-99 | 94 |
| EDTA plasma(n=8) | 91-103 | 96 |
| Cell culture media(n=8) | 97-109 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 10.82 | 30.90 | 84.42 | 10.62 | 30.48 | 90.49 |
| Standard deviation | 1.17 | 2.70 | 6.42 | 1.18 | 2.15 | 6.42 |
| CV (%) | 10.81 | 8.74 | 7.60 | 11.11 | 7.05 | 7.09 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
