Rat DIO2 (Deiodinase, Iodothyronine, Type II) Sandwich ELISA Kit Ready-To-Use Kit (STJE0014816)
SPECIFICATIONS
General Information
| Short Description | This DIO2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 0.24ng/mL |
| Detection Limit | 0.781-50ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Dio2 |
| Gene ID | 65162 |
| Uniprot ID | IOD2_RAT |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in cerebral cortex, cerebellum, pituitary gland, mostly in anterior pituitary gland, and pineal gland, as well as in brown adipose tissue (BAT). |
| Post Translational Modifications | Ubiquitinated by MARCHF6, leading to its degradation by the proteasome. Deubiquitinated by USP20 and USP33. |
| Function | Plays a crucial role in the metabolism of thyroid hormones (TH) and has specific roles in TH activation and inactivation by deiodination. Catalyzes the deiodination of L-thyroxine (T4) to 3,5,3'-triiodothyronine (T3) and 3',5'-diiodothyronine (3',5'-T2) to 3'-monoiodothyronine (3'-T1) via outer-ring deiodination (ORD). Catalyzes the deiodination of 3,3',5'-triiodothyronine (rT3) to 3,3'-diiodothyronine (3,3'-T2) via ORD. Catalyzes the phenolic ring deiodinations of 3,3',5'-triiodothyronamine and 3',5'- diiodothyronamine. |
| Protein Name | Type Ii Iodothyronine Deiodinase5diiDioiiType 2 DiType-Ii 5'-Deiodinase |
| Database Links | Reactome: R-RNO-350864 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Iii Membrane Protein |
| Alternative ELISA Names | Type Ii Iodothyronine Deiodinase ELISA kit5dii ELISA kitDioii ELISA kitType 2 Di ELISA kitType-Ii 5'-Deiodinase ELISA kitDio2 ELISA kitItdi2 ELISA kitTxdi2 ELISA kit |
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Information sourced from Uniprot.org