| Applications: |
CLIA |
| Reactivity: |
Rat |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This rat CXCR3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
Cxcr3 |
| Gene ID: |
84475 |
| Uniprot ID: |
CXCR3_RAT |
| Specificity: |
This kit recognizes Rat CXCR3 in samples. No significant cross-reactivity or interference between Rat CXCR3 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding. N-glycosylated. |
| Function | Receptor for the C-X-C chemokine CXCL9, CXCL10 and CXCL11 and mediates the proliferation, survival and angiogenic activity of mesangial cells through a heterotrimeric G-protein signaling pathway. Binds to CCL21. Probably promotes cell chemotaxis response. |
| Protein Name | C-X-C Chemokine Receptor Type 3Cxc-R3Cxcr-3Interferon-Inducible Protein 10 ReceptorIp-10 ReceptorCd Antigen Cd183 |
| Database Links | Reactome: R-RNO-380108Reactome: -RNO-418594 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative CLIA Names | C-X-C Chemokine Receptor Type 3 CLIA kitCxc-R3 CLIA kitCxcr-3 CLIA kitInterferon-Inducible Protein 10 Receptor CLIA kitIp-10 Receptor CLIA kitCd Antigen Cd183 CLIA kitCxcr3 CLIA kit |
| Specificity | This kit recognizes Rat CXCR3 in samples. No significant cross-reactivity or interference between Rat CXCR3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 90-102 | 97 |
| EDTA plasma(n=8) | 87-98 | 92 |
| Cell culture media(n=8) | 95-112 | 102 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 95.08 | 278.50 | 930.30 | 102.10 | 277.96 | 944.41 |
| Standard deviation | 10.08 | 21.89 | 84.29 | 11.61 | 29.44 | 103.41 |
| CV (%) | 10.60 | 7.86 | 9.06 | 11.37 | 10.59 | 10.95 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
