Host: | HEK293 cells |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Determination Method: | <0.1 EU/Mu g |
Immunoreactivity: | Measured by its binding ability in a functional ELISA. Immobilized Rat PD-L1 at 10 Mu g/mL (100 Mu L/well) can bind Biotinylated Rat PD-1 with a linear range of 0.0195-2.16 Mu g/mL. |
Immunogen Region: | Ala18-Thr238 |
Immunogen: | Recombinant Rat B7-H1/PD-L1/CD274 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ala18-Thr238) of rat B7-H1/PD-L1/CD274 (Accession #NP_001178883.1) fused with a 6×His tag at the C-terminus. |
Immunogen Sequence: | AFTITAPKDLYVVEYGSNVT MECRFPVEQKLDLLALVVYW EKEDKEVIQFVEGEEDLKPQ HSSFRGRAFLPKDQLLKGNA VLQITDVKLQDAGVYCCMIS YGGADYKRITLKVNAPYRKI NQRISMDPATSEHELMCQAE GYPEAEVIWTNSDHQSLSGE TTVTTSQTEEKLLNVTSVLR VNATANDVFHCTFWRVHSGE NHTAELIIPELPVPRLPHNR T |