| Function | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. |
| Protein Name | AcetylcholinesteraseAche |
| Cellular Localisation | SynapseSecretedCell MembranePeripheral Membrane ProteinIsoform H: Cell MembraneLipid-AnchorGpi-AnchorExtracellular Side |
| Alternative ELISA Names | Acetylcholinesterase ELISA kitAche ELISA kitAche ELISA kit |
| Specificity | This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 92-103 | 97 |
| EDTA plasma (n=8) | 89-99 | 94 |
| Cell culture media (n=8) | 90-106 | 97 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.43 | 7.13 | 17.70 | 2.43 | 7.84 | 16.22 |
| Standard deviation | 0.13 | 0.29 | 0.91 | 0.13 | 0.42 | 0.57 |
| CV (%) | 5.35 | 4.07 | 5.14 | 5.35 | 5.36 | 3.51 |
