| Tissue Specificity | Has been found in central nervous system and muscle. Found in embryonic liver and spleen but not in adult liver. |
| Function | Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. |
| Protein Name | AcetylcholinesteraseAche |
| Cellular Localisation | SynapseSecretedCell MembranePeripheral Membrane ProteinIsoform H: Cell MembraneLipid-AnchorGpi-AnchorExtracellular Side |
| Alternative CLIA Names | Acetylcholinesterase CLIA kitAche CLIA kitAche CLIA kit |
| Specificity | This kit recognizes Rat AChE in samples. No significant cross-reactivity or interference between Rat AChE and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-106 | 100 |
| EDTA plasma(n=8) | 88-99 | 93 |
| Cell culture media(n=8) | 94-107 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.54 | 1.25 | 4.99 | 0.53 | 1.27 | 4.71 |
| Standard deviation | 0.05 | 0.09 | 0.51 | 0.07 | 0.09 | 0.49 |
| CV (%) | 9.26 | 7.20 | 10.22 | 13.21 | 7.09 | 10.40 |
