Rat ACAT1 (Acetyl Coenzyme A Acetyltransferase 1) Sandwich ELISA Kit Ready-To-Use Kit (STJE0013212)
SPECIFICATIONS
General Information
| Short Description | This ACAT1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in rat tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Rat |
| Sensitivity | 12.5pg/mL |
| Detection Limit | 31.2-2000pg/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Acat1 |
| Gene ID | 25014 |
| Uniprot ID | THIL_RAT |
| Immunogen Region | Ready-To-Use |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | |
| Post Translational Modifications | Succinylation at Lys-265, adjacent to a coenzyme A binding site. Desuccinylated by SIRT5. |
| Function | This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism. |
| Protein Name | Acetyl-Coa Acetyltransferase - MitochondrialAcetoacetyl-Coa Thiolase |
| Database Links | Reactome: R-RNO-70895Reactome: -RNO-77108Reactome: -RNO-77111Reactome: -RNO-9837999Reactome: -RNO-9854311 |
| Cellular Localisation | Mitochondrion |
| Alternative ELISA Names | Acetyl-Coa Acetyltransferase - Mitochondrial ELISA kitAcetoacetyl-Coa Thiolase ELISA kitAcat1 ELISA kit |
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Information sourced from Uniprot.org