| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
RASGRF2 Positive Control for STJ502753 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
RASGRF2 |
| Gene ID: |
5924 |
| Uniprot ID: |
RGRF2_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with RASGRF2 antibody STJ502753. |
| Tissue Specificity | Widely expressed with higher expression in brain, followed by heart, lung, pancreas and kidney. Detected in placenta. Expressed in brain and lung (at protein level). |
| Post Translational Modifications | Phosphorylated by CDK5.down-regulates RASGRF2-mediated RAC1 activation. Ubiquitinated upon interaction with Ras. Ubiquitination leads to degradation through the 26S proteasome. |
| Function | Functions as a calcium-regulated nucleotide exchange factor activating both Ras and RAC1 through the exchange of bound GDP for GTP. Preferentially activates HRAS in vivo compared to RRAS based on their different types of prenylation. Functions in synaptic plasticity by contributing to the induction of long term potentiation. |
| Peptide Name | Ras-Specific Guanine Nucleotide-Releasing Factor 2Ras-Grf2Ras Guanine Nucleotide Exchange Factor 2 |
| Database Links | Reactome: R-HSA-193648Reactome: R-HSA-416482Reactome: R-HSA-442982Reactome: R-HSA-5673001Reactome: R-HSA-8980692Reactome: R-HSA-9013148Reactome: R-HSA-9013149 |
| Cellular Localisation | CytoplasmCell MembranePeripheral Membrane ProteinEndoplasmic Reticulum MembraneTranslocates To Membranes When ActivatedFound Both At Cell Periphery And Along The Axon Of Neurons |
| Alternative Peptide Names | Ras-Specific Guanine Nucleotide-Releasing Factor 2 proteinRas-Grf2 proteinRas Guanine Nucleotide Exchange Factor 2 proteinRASGRF2 proteinGRF2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
