Monoclonal Antibody | Primary Antibodies | St John's Laboratory

Western blot analysis of lysates from Recombinant Mouse EGF Protein (STJP002883) using EGF Rabbit mAb (STJ11107369) at 1:1000 dilution incubated overnight at 4C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 5ng, 1ng,0. 1ng Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 20s.

Anti-EGF antibody (977-1029 aa) [SRM] (STJ11107369)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

$174.83 - $775.58

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Western blot analysis using EGF monoclonal antibody against EGF-hIgGFc transfected HEK293 cell lysate.

Anti-EGF antibody [4E11] (STJ98013)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ELISA

Reactivity :

Human

$194.40 - $469.80

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Standard Curve for Human EGF: Capture Antibody Mouse mAb 201165-5E9 (5E9-A10-A6) to Human EGF at 4 Mu g/ml and Detector Antibody Mouse mAb 201165-7C11 (7C11-G10-H8) to Human EGF at 1 Mu g/ml.

Anti-EGF antibody [7C11-G10-H8] (STJ99272)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

$194.40 - $469.80

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Anti-eGFP antibody [SM5111] (STJA0005111)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF

Reactivity :

eGFP

$124.88

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Immunocytochemistry analysis of EGFR (green) in 3T3 using EGFR antibody, and DAPI (blue).

Anti-EGFR antibody (1075-1093 aa) [R01-2D3] (STJA0012677)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse

$357.08

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Immunocytochemistry analysis of EGFR (green) in Hela using EGFR antibody, and DAPI (blue)

Anti-EGFR antibody (1075-1093 aa) [R03-6B1] (STJA0013018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse

$357.08

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Anti-EGFR antibody (1075-1093 aa) [R09-7I5] (STJA0011260)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

$332.78

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Human appendix tissue was stained with Anti-EGFR (PTR2121) Antibody

Anti-EGFR antibody (300-400) [PTR2121] (STJA0006243)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$64.80 - $340.20

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Western blot analysis of lysates from wild type (WT) and EGFR knockdown (KD) HeLa cells, using [KD Validated] EGFR Rabbit monoclonal antibody (STJ11105117) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EGFR antibody (320-420 aa) [S5117RM] (STJ11105117)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma stained with anti-EGFR antibody using peroxidase-conjugate and DAB chromogen. Note the membrane staining of carcinoma cells

Anti-EGFR antibody (344-492aa) [ZR16] (STJ180567)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

$230.18 - $843.08

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Anti-EGFR antibody (800-900) [S9MR] (STJ11102229)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded Carcinoma of Human lung tissue using EGFR antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EGFR antibody (900-1210 aa) [1B10] (STJA0013541)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

$394.88

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Anti-EGFR antibody [2+00E+09] (STJ16100028)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IF/IP/EM

Reactivity :

Human

$342.23

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Western blot detection of EGFR in HCC827, A549 and MDA-MB-468 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [3F12-1H7-A10] (STJ99228)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC/IP

Reactivity :

Human/Monkey

$194.40 - $469.80

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Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.

Anti-EGFR antibody [5E10-G10-E11] (STJ99229)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IP

Reactivity :

Human/Monkey

$194.40 - $469.80

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Western blot analysis of 1) A431, 2) Hela, diluted at 1:2000.

Anti-EGFR antibody [M6] (STJ97036)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$64.80 - $340.20

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Western blot analysis of EGFR at dilution of 1:1000

Anti-EGFR antibody [SM4913] (STJA0004913)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$230.18 - $315.23

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Western blot analysis of EGFR at dilution of 1:2000

Anti-EGFR antibody [SM4954] (STJA0004954)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

$230.18 - $315.23

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Anti-EGFR antibody [SRM] (STJ11106993)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$197.78 - $867.38

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Western blot analysis of extracts from normal (control) and PHD2/EGLN1 knockout (KO) 293T (KO) cells, using PHD2/EGLN1 antibody (STJ11104243) at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 8s.

Anti-EGLN1 antibody (1-100) [S4243RM] (STJ11104243)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of extracts of various cell lines, using Prolyl hydroxylase PHD1 (EGLN2) Rabbit monoclonal antibody (STJ11101940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-EGLN2 antibody (1-100) [S0MR] (STJ11101940)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of PHD1/prolyl hydroxylase in rat heart lysates using PHD1 antibody.

Anti-EGLN2 antibody (21-34 aa) [R01-7G1] (STJA0012658)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

$357.08

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PHD3 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EGLN3 antibody (1-239 aa) [R04-3K9] (STJA0011783)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

$332.78

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Western blot analysis using Egr-1 monoclonal antibody against EGR1-hIgGFc transfected HEK293 (1) cell lysate.

Anti-EGR1 antibody (282-433 aa) [8A6] (STJ98014)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of lysates from 293T cells using EGR1 Rabbit mAb (STJ11107309) at 1:1000 dilution incubated overnight at 4C. 293T cells were treated by 10% FBS at 37C for 30 minutes after serum-starvation overnight. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 30s.

Anti-EGR1 antibody (90-250 aa) [SRM] (STJ11107309)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse brain cells using 3 Mu g EGR2 antibody (STJ11101839). Western blot was performed from the immunoprecipitate using EGR2 antibody (STJ11101839) at a dilution of 1:500.

Anti-EGR2 antibody (1-100) [S9MR] (STJ11101839)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded human appendix using EHD1 Rabbit monoclonal antibody (STJ11101885) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-EHD1 antibody (1-100) [S5MR] (STJ11101885)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunoprecipitation of EHF from 500 µg extracts of LNCaP cells was performed using 2 µg of EHF Rabbit mAb (STJ11107330). Rabbit IgG isotype control (STJ11105763) was used to precipitate the Control IgG sample. IP samples were eluted with 1X Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using EHF Rabbit mAb (A24643) at a dilution of 1 : 1000.

Anti-EHF antibody (1-113 aa) [SRM] (STJ11107330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis using EHMT1 monoclonal antibody against EHMT1 recombinant protein.

Anti-EHMT1 antibody [1B7B4] (STJ98015)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of extracts of A375 cells, using EHMT2 antibody (STJ11103018) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EHMT2 antibody (2-300) [S8MR] (STJ11103018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various lysates, using eIF1A Rabbit monoclonal antibody (STJ11104132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EIF1AX antibody (65-144) [S4132RM] (STJ11104132)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $775.58

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Immunocytochemistry analysis of eIF1A (green) in 293 using eIF1A antibody, and DAPI (blue).

Anti-EIF1AX antibody (97-110 aa) [R04-8B7] (STJA0011264)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$332.78

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Western blot analysis of various lysates using eIF2A Rabbit mAb (STJ11102530) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-EIF2A antibody (450-550) [S0MR] (STJ11102530)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunocytochemistry analysis of eIF2A (green) in MCF-7 using eIF2A antibody, and DAPI (blue)

Anti-EIF2A antibody (480-494 aa) [R08-8E2] (STJA0011265)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$357.08

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Western blot analysis of lysates from wild type (WT) and EIF2AK1 knockdown (KD) 293T cells, using EIF2AK1 Rabbit monoclonal antibody (STJ11105417) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF2AK1 antibody (337-586) [S5417RM] (STJ11105417)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of extracts of various cell lines, using PKR/EIF2AK2 antibody (STJ11101690) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK2 antibody (450-551) [S0MR] (STJ11101690)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis using PERK monoclonal antibody against HEK293 (1) and EIF2AK3-hIgGFc transfected HEK293 (2) cell lysate.

Anti-EIF2AK3 antibody [5G5] (STJ98319)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of various lysates, using GCN2 rabbit monoclonal antibody (STJ11101809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-EIF2AK4 antibody (1-300) [S9MR] (STJ11101809)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of various lysates using GCN2 Rabbit monoclonal antibody (STJ11105120) at 1：1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-EIF2AK4 antibody [S5120RM] (STJ11105120)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $775.58

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Western blot analysis of various lysates using eIF2B epsilon (EIF2B5) (EIF2B5) Rabbit mAb (STJ11102350) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-EIF2B5 antibody (1-100) [S0MR] (STJ11102350)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using eIF2C3 Rabbit monoclonal antibody (STJ11103159) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-eIF2C3 antibody (721-860) [S9MR] (STJ11103159)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot detection of eIF2 Alpha in K562, COS7, 3T3, C2C12, MCF-7, C6 and Hela cell lysates using eIF2 Alpha mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.

Anti-EIF2S1 antibody [2E4-F5-E7] (STJ99266)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human/Mouse/Rat

$194.40 - $469.80

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Western blot analysis of EIF2A at dilution of 1:1000

Anti-EIF2S1 antibody [SM5103] (STJA0005103)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$230.18 - $315.23

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Western blot analysis of various lysates using eIF3B Rabbit mAb (STJ11102435) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-EIF3B antibody (101-200) [S5MR] (STJ11102435)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunohistochemistry analysis of paraffin-embedded mouse testis using eIF3e Rabbit monoclonal antibody (STJ11101868) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-EIF3E antibody (210-350) [S8MR] (STJ11101868)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EIF4A1 antibody [M8] (STJ97038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunoprecipitation analysis of 600 Mu g extracts of Mouse testis using 3 Mu g eIF4E antibody (STJ11101582). Western blot was performed from the immunoprecipitate using eIF4E (STJ11101582) at a dilution of 1:500.

Anti-EIF4E antibody (118-217) [S2MR] (STJ11101582)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Mouse/Rat

$174.83 - $775.58

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Western blot analysis using eIF4E monoclonal antibody against HeLa (1) , HEK293 (2) and K562 (3) cell lysate.

Anti-EIF4E antibody [5D11] (STJ98016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

$194.40 - $469.80

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Monoclonal Antibodies