Anti-VIM antibodies

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S39 Rabbit pAb (STJ117904) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S39 antibody (STJ117904)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S56 pAb (STJ117902) at 1:3000 dilution or Vimentin antibody (STJ249342). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. HeLa cells were treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S56 antibody (STJ117902)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (STJ11101153) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37 °C for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ11101153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 pAb (STJ117897) at 1:2000 dilution or Vimentin antibody (STJ26092). HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-VIM-S83 antibody (STJ117897)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £642.50

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Anti-Phospho-VIM-Ser39 antibody (STJ196472)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of A549 cells using Phospho-Vimentin (S56) Polyclonal Antibody

Anti-Phospho-VIM-Ser56 antibody (31-80 aa) (STJ90927)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 293 cells using Phospho-Vimentin (S83) Polyclonal Antibody

Anti-Phospho-VIM-Ser83 antibody (56-105 aa) (STJ90926)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HELA cells using Antibody diluted at 500. Secondary antibody was diluted at 1:20000

Anti-Phospho-VIM-Tyr38 antibody (STJ98572)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysate from Jurkat cells, using phospho-Vimentin (Phospho-Tyr61) antibody.

Anti-Phospho-VIM-Tyr61 antibody (STJ90435)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-Phospho-Vimentin-S34 antibody [YT33] (STJA0019156)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Vimentin-S56 antibody [4A4] (STJA0019155)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Phospho-Vimentin-S83 antibody [MO82] (STJA0019157)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

£378.50 - £1,632.50

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Anti-Recombinant-Phospho-VIM/Vimentin-Ser56 antibody [RM1L74] (STJA0023662)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human

£309.50 - £472.50

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Anti-Recombinant-Phospho-VIM/Vimentin-Ser72 antibody [RM1L75] (STJA0023663)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Rat

£309.50 - £472.50

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Anti-Recombinant-VIM/Vimentin antibody [RM1L76] (STJA0023664)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat/Hamster

£309.50 - £472.50

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Western blot analysis of various lysates using Vimentin Rabbit pAb (STJ26092) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-VIM antibody (1-200) (STJ26092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit polyclonal antibody (STJ113868) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (1-466) (STJ113868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunofluorescence analysis of U-2 OS cells using Vimentin Rabbit polyclonal antibody (STJ11104460) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-VIM antibody (10-101) (STJ11104460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of Vimentin in paraffin-embedded rat spleen using Vimentin Rabbit polyclonal antibody (STJ113129) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-VIM antibody (300-400) (STJ113129)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-VIM antibody (367-466) [S7MR] (STJ11101727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Anti-VIM antibody at 1:2500 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-VIM antibody (390aa C-Term) (STJ140133)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Human appendix tissue was stained with Anti-Vimentin (ABT019) Antibody

Anti-VIM antibody (400-466 aa) [ABT019] (STJ197249)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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U2OS whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Vimentin (ABT281) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: U2OSPredicted band size: 54kDaObserved band size: 54kDa

Anti-VIM antibody (400-466 aa) [ABT281] (STJ197198)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Immunocytochemistry analysis of Vimentin (green) in Hela using Vimentin antibody, and DAPI (blue).

Anti-VIM antibody (448-465 aa) [R02-8C4] (STJA0013017)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat/Hamster

£264.50

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Immunofluorescence analysis of Hela cell. 1, Vimentin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-VIM antibody (56-105 aa) (STJ96245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain tissue, diluted at 1:2000.

Anti-VIM antibody [1A7] (STJ97060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Vimentin in Molt-4, K562, COS7, Jurkat, Hela and Vimentin negative cell (Daudi, Ramos, Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.

Anti-VIM antibody [4F8-A5-H5] (STJ99270)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IP

Reactivity :

Human

£144.00 - £348.00

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Anti-VIM/Vimentin antibody [SAA0112] (STJA0026948)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-VIM/Vimentin antibody [SAA0112] {APC} (STJA0029094)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-VIM/Vimentin antibody [SAA0112] {FITC} (STJA0028128)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£254.50 - £378.50

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Anti-VIM/Vimentin antibody [SAA0112] {PerCP} (STJA0030060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£292.50 - £440.50

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Anti-VIM/Vimentin antibody [SAA0112] {PE} (STJA0031026)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC

Reactivity :

Human

£310.50 - £472.50

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Anti-VIM/Vimentin antibody [VB6] (STJA0026947)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-VIM/Vimentin antibody [VB6] {APC} (STJA0029093)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£310.50 - £472.50

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Anti-VIM/Vimentin antibody [VB6] {FITC} (STJA0028127)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£254.50 - £378.50

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Anti-VIM/Vimentin antibody [VB6] {PerCP} (STJA0030059)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£292.50 - £440.50

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Anti-VIM/Vimentin antibody [VB6] {PE} (STJA0031025)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/FC/WB

Reactivity :

Human

£310.50 - £472.50

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Formalin-fixed, paraffin-embedded human gastrointestinal stromal tumor stained with anti-vimentin antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of tumor cells

Anti-Vimentin antibody (2-466aa) [ZR381] (STJ180548)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£161.50 - £496.50

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STJ73113 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Vimentin antibody (C-Term) (STJ73113)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/FC/IF/IHC

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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Immunostaining of tumors and precursor PanIN lesions from 4 week old LSL-KrasG12D; Cdkn2alox/lox; p48Cre (KIC) mice that were treated with 2G8 and/or gemcitabine, Gem. (Anti-Vimentin, cat. STJA0003823, green; Anti-E-cadherin, red). Photo courtesy of Rolf Brekken, University of Texas Southwestern. CC-BY-4. 0

Anti-Vimentin antibody (STJA0003823)

Clonality :

Polyclonal

Host :

Chicken

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Vole

£403.50

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~50 kDa vimentin protein.

Anti-Vimentin antibody [2D1] (STJA0003824)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Chicken/D.melanogaster/Feline/Finch/Fish/Goat/Guinea Pig/Hamster/Horse/Lizard/Non-Human Primates/Rabbit/Sheep/Vole/Xenopus/Zebrafish

£454.50

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Anti-Vimentin antibody [65E] (STJ16100819)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC-F/WB

Reactivity :

Human

£606.50

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Anti-Vimentin antibody [EBS-O-184] (STJ16102604)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Human

£307.50 - £1,449.50

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Anti-Vimentin antibody [LN-6] (STJ16101027)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/IF/IHC

Reactivity :

Cat/Cow/Human/Mouse/Pig/Rabbit/Rat/Sheep

£307.50 - £1,449.50

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Anti-Vimentin antibody [LN6] (STJA0003872)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human

£454.50

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Anti-Vimentin antibody [SM0430] (STJA0010430)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

£170.50 - £233.50

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Western blot analysis of Vimentin. Sample: Protein treated by RIPA Lysis Buffer. Blocking buffer: 3% Nonfat dry milk in TBST, RT, 1h. Primary antibody: 1:1000, 4C overnight. Secondary antibody: HRP Goat Anti-mouse lgG, 1:3000, RT, 1h.

Anti-Vimentin antibody [SM0448] (STJA0010448)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£170.50 - £233.50

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Anti-Vimentin antibody [SM5190] (STJA0005190)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF

Reactivity :

Human

£233.50

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VIM antibodies

Target Information

Synonyms