Immunofluorescence Antibodies | St John's Laboratory

Immunofluorescence analysis of U-2 OS cells using [KO Validated] Galectin 3/LGALS3 Rabbit polyclonal antibody (STJ115467) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LGALS3 antibody (1-250) (STJ115467)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of Hela cell. 1, CBP 35 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-LGALS3 antibody (141-190 aa) (STJ92060)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of 1) MCF7, 2) 3T3 with Galectin-3 Mouse mAb diluted at 1:2, 000.

Anti-LGALS3 antibody [8D7] (STJ97550)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-LGALS3 antibody [P3] (STJA0027508)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rabbit

£315.50

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Anti-LGALS3 antibody [P3] {APC} (STJA0029244)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£391.50

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Anti-LGALS3 antibody [P3] {FITC} (STJA0028278)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£315.50

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Anti-LGALS3 antibody [P3] {PerCP} (STJA0030210)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£365.50

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Anti-LGALS3 antibody [P3] {PE} (STJA0031176)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC/IF/WB

Reactivity :

Human/Mouse/Rabbit

£391.50

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Immunofluorescence analysis of HeLa cells using JNK1 antibody (STJ24490) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MAPK8 antibody (245-345) (STJ24490)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot detection of JNK1 in C6 and 3T3 cell lysates using JNK1 mouse mAb (1:1000 diluted).Predicted band size:46, 54KDa.Observed band size:46KDa.

Anti-MAPK8 antibody [1A4-C5-F11] (STJ99225)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF

Reactivity :

Human/Mouse/Rat

£149.00 - £360.00

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Western blot analysis of lysates from HeLa cells using cGAS Rabbit pAb (STJ110633) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MB21D1 antibody (350-522) (STJ110633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70753 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-MEFV antibody (Internal) (STJ70753)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of lysates from Jurkat and 293 cells, using Phospho-Bcl-2-S70 Rabbit pAb (STJ29341) at 1:1000 dilution. Jurkat cells were treated by serum-starvation overnight. 293 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-BCL2-S70 antibody (STJ29341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£126.50 - £618.50

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Western blot analysis of various cells using Phospho-Bcl-2 (S70) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-BCL2-Ser70 antibody (41-90 aa) (STJ90191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Monkey

£49.00 - £261.00

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Western blot analysis of 293T-UV cells using Phospho-Bcl-2 (S87) Polyclonal Antibody diluted at 1：500

Anti-Phospho-BCL2-Ser87 antibody (61-110 aa) (STJ90806)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various cells using Phospho-Bcl-2 (T56) Polyclonal Antibody diluted at 1：500

Anti-Phospho-BCL2-Thr56 antibody (26-75 aa) (STJ90192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using BCL-2 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-BCL2-Thr69 antibody (46-95 aa) (STJ90193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human meningioma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-BCL2-Thr74 antibody (STJ91281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-DAPK1-Ser308 antibody (274-323 aa) (STJ91301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-DAPK1-Ser308 antibody (STJ193210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse

£49.00 - £261.00

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Immunofluorescence analysis of rat-testis tissue. 1, JNK1/2/3 (phospho Thr183) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-JNK1/2/3-Thr183 antibody (151-200 aa) (STJ90316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

£49.00 - £261.00

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Immunofluorescence analysis of rat-liver tissue. 1, JNK1/2/3 (phospho Thr183/Y185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-JNK1/2/3-Thr183/Y185 antibody (151-200 aa) (STJ90317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken/Pig/Fish

£49.00 - £261.00

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Immunofluorescence analysis of human-lung tissue. 1, JNK1/2/3 (phospho Tyr185) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-JNK1/2/3-Tyr185 antibody (151-200 aa) (STJ91003)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of PC-12 cells using AMPK Alpha 2 Rabbit polyclonal antibody (STJ11103813) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-PRKAA2 antibody (270-343) (STJ11103813)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Rat

£110.50 - £505.50

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Western blot analysis of various lysates, using PRKAA2 antibody (STJ115987) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PRKAA2 antibody (270-552) (STJ115987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and AMPK Alpha 2 knockout (KO) HCT116 cells, using [KO Validated] AMPK Alpha 2 Rabbit polyclonal antibody (STJ29478) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PRKAA2 antibody (270-552) (STJ29478)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Recombinant-Bcl-2 antibody [SR0674] (STJA0010674)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse

£153.50 - £228.50

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Anti-Recombinant-DAPK1 antibody [RM1B14] (STJA0026784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Galectin 3 antibody [SM0643] (STJA0010643)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£153.50 - £228.50

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Anti-Recombinant-GRB2 antibody [RM2A58] (STJA0025032)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-GRB2 antibody [RM2A59] (STJA0025033)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-HMGB1 antibody [RM1A30] (STJA0026701)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-JNK1/2/3 antibody [RM1C15] (STJA0026884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human/Mouse/Rat/Cow/Dog/Monkey/Zebrafish

£300.50 - £457.50

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Anti-Recombinant-LGALS3/Galectin-3 antibody [RM1A61] (STJA0026732)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Anti-Recombinant-LGALS3/Galectin-3 antibody [RM1Q62] (STJA0024046)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/WB

Reactivity :

Human/Rat

£300.50 - £457.50

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Anti-Recombinant-LGALS3/Galectin-3 antibody [RM1Q64] (STJA0024048)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/WB

Reactivity :

Human/Rat/Monkey

£300.50 - £457.50

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Anti-Recombinant-LGALS3/Galectin-3 antibody [RM1Q65] (STJA0024049)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-Recombinant-MAPK8 antibody [RM1W70] (STJA0024648)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-Phospho-JNK1/2/3-T183 + T183 + T221 antibody [RM1C42] (STJA0026911)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Anti-Recombinant-USP10 antibody [RM2F70] (STJA0025539)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-Recombinant-USP10 antibody [RM2F71] (STJA0025540)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-Recombinant-USP13 antibody [RM2M05] (STJA0026068)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IF/IP/WB

Reactivity :

Human/Mouse

£300.50 - £457.50

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Anti-Recombinant-USP13 antibody [RM2M06] (STJA0026069)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/IP/WB

Reactivity :

Human

£300.50 - £457.50

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HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed with STJ70342 (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with STJ70342 (1mg/ml) in Lane B. Detected by chemiluminescence.

Anti-SSA1/Ro52 antibody (C-Term) (STJ70342)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB-Trf/IF/FC

Reactivity :

Human

£312.50

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Western blot analysis of extracts of various cell lines, using TRIM21/SS-A antibody (STJ115508) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-TRIM21 antibody (86-334) (STJ115508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from Rat lung using TRIM21/SS-A rabbit polyclonal antibody (STJ25964) at 1:1000 dilution. Secondary antibody:HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime:180s.

Anti-TRIM21 antibody (86-334) (STJ25964)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using TRIM5 Alpha monoclonal antibody against human breast carcinoma tissue lysate.

Anti-TRIM5 antibody [3B11H2] (STJ98429)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£149.00 - £360.00

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Confocal imaging of NIH/3T3 cells using USP10 Rabbit monoclonal antibody (STJ11102084, at dilution of 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-USP10 antibody (1-100) [S4MR] (STJ11102084)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£118.50 - £521.50

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