Immunocytochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using DDB2 antibody (STJ23354) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DDB2 antibody (1-315) (STJ23354)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using DDC antibody (STJ23355) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-DDC antibody (1-137) (STJ23355)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DDDDK-Tag using Rabbit anti DDDDK-Tag pAb (STJ11103696) at 1:10000 dilution. <br/>Secondary antibody: (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-DDDDK-Tag antibody (STJ11103696)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP

Reactivity :

Species Independent

£156.50 - £290.50

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Western blot analysis of lysates from wild type (WT) and 293F cells transfected with Mouse anti DDDDK-Tag using Mouse anti DDDDK-Tag mAb (STJ11103697) at 1:20000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 20 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit ( Exposure time: 45s.

Anti-DDDDK-Tag antibody [S7MM] (STJ11103697)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/IP/FC/ELISA

Reactivity :

Species Independent

£156.50 - £290.50

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Anti-DDDDK-Tag antibody [SRM15108] (STJ11109838)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/FC/ELISA/ChIP/ChIP-seq

Reactivity :

Species Independent

£0.00

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Immunofluorescence analysis of U2OS cells using DDIT3/CHOP Rabbit polyclonal antibody (STJ23356) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DDIT3 antibody (1-169) (STJ23356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of 293F, using DDX1 rabbit polyclonal antibody (STJ28658) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-DDX1 antibody (441-740) (STJ28658)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells using DDX20 antibody (STJ28380). Blue: DAPI for nuclear staining.

Anti-DDX20 antibody (25-280) (STJ28380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse testis using DDX21 antibody (STJ117020) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-DDX21 antibody (1-180) (STJ117020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunocytochemistry analysis of DDX21 (green) in 293T using DDX21 antibody, and DAPI (blue).

Anti-DDX21 antibody (248-260 aa) [R04-4J2] (STJA0011226)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human

£246.50

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Immunofluorescence analysis of U-2 OS cells using DDX24 Rabbit polyclonal antibody (STJ117676) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DDX24 antibody (510-859) (STJ117676)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-DDX3 antibody (100-200 aa) [SRM5180] (STJ11107047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of L929 cells using DDX31 Rabbit polyclonal antibody (STJ118337) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DDX31 antibody (652-851) (STJ118337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using DDX39A Rabbit pAb (STJ110264) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DDX39A antibody (1-100) (STJ110264)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using UAP56/DDX39B Rabbit pAb (STJ110654) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-DDX39B antibody (319-428) (STJ110654)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-DDX3X antibody (100-200) (STJ11105653)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using DDX3X antibody (STJ27604) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX3X antibody (17-33) (STJ27604)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of DDX3 (6G8) in HeLa using DDX3 antibody.

Anti-DDX3X antibody (22-178 aa) [6G8-F4-E3] (STJA0012848)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IF/IP/ChIP

Reactivity :

Human/Rat/Mouse/Monkey

£264.50

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Western blot detection of DDX3 in Hela, 3T3, C6, COS7, K562 and Jurkat cell lysate using DDX3 mouse mAb (1:1000 diluted).Predicted band size: 75KDa.Observed band size: 75KDa.

Anti-DDX3X antibody [6G8-F4-E3] (STJ99237)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP/ICC

Reactivity :

Human/Rat/Mouse/Monkey

£149.00 - £360.00

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Anti-DDX3X/DDX3Y antibody [SRM14684] (STJ11109414)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunofluorescence analysis of U-2 OS cells using DDX3Y Rabbit polyclonal antibody (STJ113303) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DDX3Y antibody (1-210) (STJ113303)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of mouse testis, using DDX4 antibody (STJ118072) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DDX4 antibody (635-724) (STJ118072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells using 1 Mu g DDX41 antibody (STJ28659). Western blot was performed from the immunoprecipitate using DDX41 antibody (STJ28659) at a dilution of 1:1000.

Anti-DDX41 antibody (1-300) (STJ28659)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of DDX5 (green) in Hela using DDX5 antibody, and DAPI (blue)

Anti-DDX5 antibody (9-21 aa) [R04-2K2] (STJA0011227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£246.50

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Anti-DDX5 antibody [SRM13027] (STJ11107757)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates using DDX50 Rabbit pAb (STJ113576) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-DDX50 antibody (1-135) (STJ113576)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U20S cells using Rig-I/DDX58 antibody (STJ115369) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DDX58 antibody (726-925) (STJ115369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using DDX6 Rabbit mAb (STJ11102512) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-DDX6 antibody (101-200) [S2MR] (STJ11102512)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £554.50

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Western blot analysis of extracts of various cell lines, using DDX6 antibody (STJ118705) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-DDX6 antibody (414-533) (STJ118705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Dectin-1 antibody (66-247 aa) [SRM5408] (STJ11107319)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Immunofluorescence analysis of Jurkat cells using DEFA1 Rabbit polyclonal antibody (STJ28977) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DEFA1 antibody (1-94) (STJ28977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of mouse testis cells using DEFB121 antibody (STJ23365) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DEFB121 antibody (1-76) (STJ23365)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using Defb26 antibody (STJ118348) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-DEFB126 antibody (22-128) (STJ118348)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with DEFB4A using DEFB4A Rabbit pAb (STJ11107083) at1:1000 dilution. <br>Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 20 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection:ECL Basic Kit <br>Exposuretime: 30s.

Anti-DEFB4A antibody (1-64 aa) (STJ11107083)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£110.50 - £505.50

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Immunofluorescence analysis of C6 cells using DEK Rabbit monoclonal antibody (STJ11102678) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-DEK antibody (51-200) [S8MR] (STJ11102678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Rat

£118.50 - £521.50

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Immunofluorescence analysis of paraffin-embedded rat heart using Desminmin Rabbit polyclonal antibody (STJ23369) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DES antibody (1-470) (STJ23369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of paraffin-embedded rat heart using Desmin Rabbit monoclonal antibody (STJ11101942) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DES antibody (371-470) [S2MR] (STJ11101942)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of HeLa cells using [KO Validated] DFFA Rabbit polyclonal antibody (STJ29835). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DFFA antibody (1-331) (STJ29835)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-DFos antibody (450-550) (STJ500757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

£466.50

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Anti-DFos antibody (450-550) {Biotin} (STJ500760)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

£528.50

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Anti-DFos antibody (450-550) {FITC} (STJ500761)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

£528.50

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Immunohistochemistry of third instant ventral ganglion with Anti-DFos Antibody (STJ500756) at 1:250 dilution in Buffer.

Anti-DFos antibody (5-80) (STJ500756)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

£466.50

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Anti-DFos antibody (5-80) {Biotin} (STJ500758)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

£528.50

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Anti-DFos antibody (5-80) {FITC} (STJ500759)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IP/WB

Reactivity :

D.melanogaster

£528.50

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Western blot analysis of extracts of various cell lines, using DGAT1 antibody (STJ28938) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-DGAT1 antibody (263-278) (STJ28938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunocytochemistry analysis of DGCR8 (green) in K562 using DGCR8 antibody, and DAPI (blue).

Anti-DGCR8 antibody (424-773 aa) [R05-5A6] (STJA0011234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

£264.50

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Immunofluorescence analysis of NIH/3T3 cells using DGLUCY Rabbit polyclonal antibody (STJ11105233) at a dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-DGLUCY antibody (269-616) (STJ11105233)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of A-549 cells using DHFR antibody (STJ23375).

Anti-DHFR antibody (1-187) (STJ23375)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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