| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Presenilin 1 Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: 1:500 |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Tissue Specificity | Detected in azurophile granules in neutrophils and in platelet cytoplasmic granules (at protein level). Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes. |
| Post Translational Modifications | Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12. After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis. |
| Function | Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the presence of the other members of the gamma-secretase complex for protease activity. Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels. Stimulates cell-cell adhesion via its interaction with CDH1.this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin). Under conditions of apoptosis or calcium influx, cleaves CDH1. This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling. Required for normal embryonic brain and skeleton development, and for normal angiogenesis. Mediates the proteolytic cleavage of EphB2/CTF1 into EphB2/CTF2. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is therefore involved in calcium homeostasis. Involved in the regulation of neurite outgrowth. Is a regulator of presynaptic facilitation, spike transmission and synaptic vesicles replenishment in a process that depends on gamma-secretase activity. It acts through the control of SYT7 presynaptic expression. |
| Peptide Name | Presenilin-1Ps-1Protein S182 Cleaved Into - Presenilin-1 Ntf Subunit - Presenilin-1 Ctf Subunit - Presenilin-1 Ctf12Ps1-Ctf12 |
| Database Links | Reactome: R-HSA-1251985Reactome: R-HSA-1474228Reactome: R-HSA-193692Reactome: R-HSA-205043Reactome: R-HSA-2122948Reactome: R-HSA-2644606Reactome: R-HSA-2894862Reactome: R-HSA-2979096Reactome: R-HSA-3928665Reactome: R-HSA-6798695Reactome: R-HSA-9013507Reactome: R-HSA-9013700Reactome: R-HSA-9017802 |
| Cellular Localisation | Endoplasmic ReticulumEndoplasmic Reticulum MembraneMulti-Pass Membrane ProteinGolgi Apparatus MembraneCytoplasmic GranuleCell MembraneCell ProjectionGrowth ConeEarly EndosomeEarly Endosome MembraneNeuron ProjectionAxonSynapseTranslocates With Bound Notch1 From The Endoplasmic Reticulum And/Or Golgi To The Cell SurfaceColocalizes With Cdh1/2 At Sites Of Cell-Cell ContactColocalizes With Ctnnb1 In The Endoplasmic Reticulum And The Proximity Of The Plasma MembraneAlso Present In Azurophil Granules Of NeutrophilsColocalizes With Ubqln1 In The Cell Membrane And In Cytoplasmic Juxtanuclear Structures Called Aggresomes |
| Alternative Peptide Names | Presenilin-1 proteinPs-1 proteinProtein S182 Cleaved Into - Presenilin-1 Ntf Subunit - Presenilin-1 Ctf Subunit - Presenilin-1 Ctf12 proteinPs1-Ctf12 proteinPSEN1 proteinAD3 proteinPS1 proteinPSNL1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
