Porcine GM-CSF protein (Recombinant) (His-Tag) (STJP019430)
SPECIFICATIONS
HostE.coli
ImmunogenPocrine
General Information
| Short Description | Recombinant-Porcine GM-CSF-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Bioactivity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid In Phosphate-Buffered Saline (pH7.4) |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 40 ng/ml. |
Target Information
| Accession Number | NP_999283 |
| Immunogen | Pocrine |
| Immunogen Region | 18-144aa |
| Immunogen Sequence |
Additional Info
| Background | GM-CSF, also known as granulocyte macrophage colony-stimulating factor, is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant porcine GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. |
Information sourced from Uniprot.org