| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
PDE9A Positive Control for STJ502390 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Dilution Range: |
WB: Apply 15-20 µl per lane |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
PDE9A |
| Gene ID: |
5152 |
| Uniprot ID: |
PDE9A_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with PDE9A antibody STJ502390. |
| Tissue Specificity | Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate. In brain, present in the cortex, cerebellum, and subiculum (at protein level). In heart, primarily localizes to myocytes. |
| Function | Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory. |
| Peptide Name | High Affinity Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase 9a |
| Database Links | Reactome: R-HSA-418457 |
| Cellular Localisation | Isoform Pde9a1: Cell ProjectionRuffle MembraneCytoplasmPerinuclear RegionGolgi ApparatusEndoplasmic ReticulumCell MembraneSarcolemmaIsoform Pde9a2: Cell ProjectionIsoform Pde9a3: CytoplasmIsoform Pde9a17: Cytoplasm |
| Alternative Peptide Names | High Affinity Cgmp-Specific 3' -5'-Cyclic Phosphodiesterase 9a proteinPDE9A protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
