| Applications: | WB |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Phospho-PDE4D ERK site Positive Control is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Dilution Range: | WB: 1:500 |
| Storage Instruction: | Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: | PDE4D |
| Gene ID: | 5144 |
| Uniprot ID: | PDE4D_HUMAN |
| Tissue Specificity | Expressed in colonic epithelial cells (at protein level). Widespread.most abundant in skeletal muscle. Isoform 6: Detected in brain. Isoform 8: Detected in brain, placenta, lung and kidney. Isoform 7: Detected in heart and skeletal muscle. |
| Post Translational Modifications | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation. Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. |
| Peptide Name | 3' -5'-Cyclic-Amp Phosphodiesterase 4d Dpde3 Pde43 Camp-Specific Phosphodiesterase 4d |
| Database Links | Reactome: R-HSA-180024 Reactome: R-HSA-418555 |
| Cellular Localisation | Apical Cell Membrane Cytoplasm Membrane Cytoskeleton Microtubule Organizing Center Centrosome Found In The Soluble Fraction Associated With Membranes And Associated With The Cytoskeleton And The Centrosome Colocalized With Shank2 To The Apical Membrane Of Colonic Crypt Cells |
| Alternative Peptide Names | 3' -5'-Cyclic-Amp Phosphodiesterase 4d protein Dpde3 protein Pde43 protein Camp-Specific Phosphodiesterase 4d protein PDE4D protein DPDE3 protein |
Information sourced from Uniprot.org