| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
NeuroD1 Positive Control for STJ506340 is synthetically produced from the 300-356 sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
NEUROD1 |
| Gene ID: |
4760 |
| Uniprot ID: |
NDF1_HUMAN |
| Immunogen Region: |
300-356 |
| Specificity: |
This is positive control is recommended for use in combination with NeuroD1 antibody STJ506340. |
| Post Translational Modifications | Phosphorylated. In islet cells, phosphorylated on Ser-274 upon glucose stimulation.which may be required for nuclear localization. In activated neurons, phosphorylated on Ser-335.which promotes dendritic growth. Phosphorylated by MAPK1.phosphorylation regulates heterodimerization and DNA-binding activities. Phosphorylation on Ser-266 and Ser-274 increases transactivation on the insulin promoter in glucose-stimulated insulinoma cells. |
| Function | Acts as a transcriptional activator: mediates transcriptional activation by binding to E box-containing promoter consensus core sequences 5'-CANNTG-3'. Associates with the p300/CBP transcription coactivator complex to stimulate transcription of the secretin gene as well as the gene encoding the cyclin-dependent kinase inhibitor CDKN1A. Contributes to the regulation of several cell differentiation pathways, like those that promote the formation of early retinal ganglion cells, inner ear sensory neurons, granule cells forming either the cerebellum or the dentate gyrus cell layer of the hippocampus, endocrine islet cells of the pancreas and enteroendocrine cells of the small intestine. Together with PAX6 or SIX3, is required for the regulation of amacrine cell fate specification. Also required for dendrite morphogenesis and maintenance in the cerebellar cortex. Associates with chromatin to enhancer regulatory elements in genes encoding key transcriptional regulators of neurogenesis. |
| Peptide Name | Neurogenic Differentiation Factor 1NeurodNeurod1Class A Basic Helix-Loop-Helix Protein 3Bhlha3 |
| Database Links | Reactome: R-HSA-210745Reactome: R-HSA-210746 |
| Cellular Localisation | CytoplasmNucleusIn Pancreatic Islet CellsShuttles To The Nucleus In Response To Glucose StimulationColocalizes With Nr0b2 In The Nucleus |
| Alternative Peptide Names | Neurogenic Differentiation Factor 1 proteinNeurod proteinNeurod1 proteinClass A Basic Helix-Loop-Helix Protein 3 proteinBhlha3 proteinNEUROD1 proteinBHLHA3 proteinNEUROD protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
