Myosin 1C Positive Control for STJ501852 is synthetically produced from the sequence and is suitable for use in western blot applications.
Applications
WB
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Dilution Range
WB: 1:2, 500-1:5, 000
Formulation
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is
Storage Instruction
Store at-20°C for long term storage. Avoid freeze-thaw cycles.
This is positive control is recommended for use in combination with Myosin 1C antibody STJ501852.
Additional Info
Tissue Specificity
Isoform 3 is expressed in small intestine, pancreas, brain, kidney, skin, heart muscle, testis, striated muscle, spleen, liver and lung (at protein level). Expressed in brain, testis, adrenal glands, thymus, spleen, kidney, lung, heart, cochlea and vestibule. Expressed in sensory hair cells of the inner ear. Expressed in adipocytes.
Post Translational Modifications
Isoform 2 contains a N-acetylmethionine at position 1.
Function
Myosins are actin-based motor molecules with ATPase activity. Unconventional myosins serve in intracellular movements. Their highly divergent tails bind to membranous compartments, which then are moved relative to actin filaments. Involved in glucose transporter recycling in response to insulin by regulating movement of intracellular GLUT4-containing vesicles to the plasma membrane. Component of the hair cell's (the sensory cells of the inner ear) adaptation-motor complex. Acts as a mediator of adaptation of mechanoelectrical transduction in stereocilia of vestibular hair cells. Binds phosphoinositides and links the actin cytoskeleton to cellular membranes. Isoform 3: Involved in regulation of transcription. Associated with transcriptional active ribosomal genes. Appears to cooperate with the WICH chromatin-remodeling complex to facilitate transcription. Necessary for the formation of the first phosphodiester bond during transcription initiation.
CytoplasmNucleusCell CortexCell ProjectionStereocilium MembraneCytoplasmic VesicleRuffle MembraneColocalizes With Cabp1 And Cib1 At Cell MarginMembrane Ruffles And Punctate Regions On The Cell MembraneColocalizes In Adipocytes With Glut4 At Actin-Based MembranesColocalizes With Glut4 At Insulin-Induced Ruffles At The Cell MembraneLocalizes Transiently At Cell Membrane To Region Known To Be Enriched In Pip2Activation Of Phospholipase C Results In Its Redistribution To The CytoplasmColocalizes With Rna Polymerase IiTranslocates To Nuclear Speckles Upon Exposure To Inhibitors Of Rna Polymerase Ii TranscriptionIsoform 3: NucleusNucleolusNucleoplasmColocalizes With Rna Polymerase Ii In The NucleusColocalizes With Rna Polymerase I In NucleoliIn The NucleolusIs Localized Predominantly In Dense Fibrillar Component (Dfc) And In Granular Component (Gc)Accumulates Strongly In Dfc And Gc During Activation Of TranscriptionColocalizes With Transcription SitesColocalizes In The Granular Cortex At The Periphery Of The Nucleolus With Rps6Colocalizes In Nucleoplasm With Rps6 And Actin That Are In Contact With Rnp ParticlesColocalizes With Rps6 At The Nuclear Pore Level
Alternative Peptide Names
Unconventional Myosin-Ic proteinMyosin I Beta proteinMmi-Beta proteinMmib proteinMyo1c protein
