| Applications: |
Immunodepletion/Immunocompetition |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
MSX1 Blocking Peptide for STJ501812 is synthetically produced from the 46-64 sequence and is suitable for use in western blot applications. |
| Formulation: |
Liquid form at 2.5mg/ml concentration in PBS. Up to 5% DMSO can be added. Orders with >1mg can be supplied in lyophilized powder form, or in buffer of choice. |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
MSX1 |
| Gene ID: |
4487 |
| Uniprot ID: |
MSX1_HUMAN |
| Immunogen Region: |
46-64 |
| Specificity: |
This blocking peptide is recommended for use in combination with MSX1 antibody, STJ501812 |
| Immunogen: |
Synthetic peptide: DEEGAKPKVSPSLLPFSVE corresponding to amino acids 46-64 of Mouse Msx1 protein. The peptide was post synthetically modified and conjugated to KLH using hetero bifunctional cross linker for immunization of rabbits. |
| Post Translational Modifications | Sumoylated by PIAS1, desumoylated by SENP1. Sumoylation of Lys-15 and Lys-133 not required for interaction with H1-5, transcriptional repression, inhibition of myoblast differentiation, or binding to gene promoters. |
| Function | Acts as a transcriptional repressor. Capable of transcription autoinactivation. Binds to the consensus sequence 5'-C/GTAAT-3' in downstream activin regulatory elements (DARE) in the gene promoter, thereby repressing the transcription of CGA/alpha-GSU and GNRHR. Represses transcription of myoblast differentiation factors. Binds to core enhancer regions in target gene promoters of myoblast differentiation factors with binding specificity facilitated by interaction with PIAS1. Recruits histone H3 methyltransferases such as EHMT2/G9a to gene promoter regions which leads to inhibition of myoblast differentiation via transcriptional repression of differentiation factors. Regulates, in a stage-specific manner, a developmental program of gene expression in the fetal tooth bud that controls odontoblast differentiation and proliferation of dental mesenchymal cells. At the bud stage, required for mesenchymal molar tooth bud development via facilitating reciprocal signaling between dental epithelial and mesenchymal cells. May also regulate expression of Wnt antagonists such as DKK2 and SFPR2 in the developing tooth mesenchyme. Required for BMP4 expression in dental mesenchyme cells. Also, in response to BMP4, required for BMP4 expression in neighboring dental epithelial cells. Required for maximal FGF4-induced expression of SDC1 in dental mesenchyme cells. Also in response to SDC1, required for SDC1 expression in neighboring dental epithelial cells. At the early bell stage, acts to drive proliferation of dental mesenchyme cells, however during the late bell stage acts as an homeostatic regulator of the cell cycle. Regulates proliferation and inhibits premature mesenchymal odontogenesis during the bell stage via inhibition of the Wnt signaling component CTNNB1 and subsequent repression of the odontoblast differentiation factors BMP2, BMP4, LEF1, ALPL and BGLAP/OCN. Additionally, required for correct development and fusion of the palatal shelves and embryonic mandibular formation. Plays a role in embryonic bone formation of the middle ear, skull and nasal bones. Required for correct formation and thickness of the nail plate. May play a role in limb-pattern formation. |
| Peptide Name | Homeobox Protein Msx-1Homeobox Protein Hox-7Msh Homeobox 1-Like Protein |
| Cellular Localisation | NucleusInteraction With Ehmt2/G9a Is Required For Localization To The Nuclear PeripheryInteraction With Pias1 Is Required For Localization To The Nuclear Periphery |
| Alternative Peptide Names | Homeobox Protein Msx-1 proteinHomeobox Protein Hox-7 proteinMsh Homeobox 1-Like Protein proteinMSX1 proteinHOX7 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
