| Applications: |
WB |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
MST3 Positive Control for STJ506032 is synthetically produced from the sequence and is suitable for use in western blot applications. |
| Formulation: |
Provided as 100 uL ready-to-use, in SDS-PAGE sample buffer (Laemelli's buffer) containing Tris, pH 6.8, 1 % SDS, Glycerol and Bromophenolblue blue as tracking dye. The sample is reduced by adding 2% beta mercaptoethanol. The protein concentration is |
| Storage Instruction: |
Store at-20°C for long term storage. Avoid freeze-thaw cycles. |
| Gene Symbol: |
STK24 |
| Gene ID: |
8428 |
| Uniprot ID: |
STK24_HUMAN |
| Specificity: |
This is positive control is recommended for use in combination with MST3 antibody STJ506032. |
| Immunogen: |
Purified Mammalian STE20-like protein kinase 3/STE20-like kinase MST3/STK24. |
| Tissue Specificity | Isoform A is ubiquitous. Isoform B is expressed in brain with high expression in hippocampus and cerebral cortex. |
| Post Translational Modifications | Proteolytically processed by caspases during apoptosis. Proteolytic cleavage results in kinase activation, nuclear translocation of the truncated form (MST3/N) and the induction of apoptosis. Isoform B is activated by phosphorylation by PKA. Oxidative stress induces phosphorylation. Activated by autophosphorylation at Thr-190 and phosphorylation at this site is essential for its function. Manganese, magnesium and cobalt-dependent autophosphorylation is mainly on threonine residues while zinc-dependent autophosphorylation is on both serine and threonine residues. |
| Function | Serine/threonine-protein kinase that acts on both serine and threonine residues and promotes apoptosis in response to stress stimuli and caspase activation. Mediates oxidative-stress-induced cell death by modulating phosphorylation of JNK1-JNK2 (MAPK8 and MAPK9), p38 (MAPK11, MAPK12, MAPK13 and MAPK14) during oxidative stress. Plays a role in a staurosporine-induced caspase-independent apoptotic pathway by regulating the nuclear translocation of AIFM1 and ENDOG and the DNase activity associated with ENDOG. Phosphorylates STK38L on 'Thr-442' and stimulates its kinase activity. In association with STK26 negatively regulates Golgi reorientation in polarized cell migration upon RHO activation. Regulates also cellular migration with alteration of PTPN12 activity and PXN phosphorylation: phosphorylates PTPN12 and inhibits its activity and may regulate PXN phosphorylation through PTPN12. May act as a key regulator of axon regeneration in the optic nerve and radial nerve. |
| Peptide Name | Serine/Threonine-Protein Kinase 24Mammalian Ste20-Like Protein Kinase 3Mst-3Ste20-Like Kinase Mst3 Cleaved Into - Serine/Threonine-Protein Kinase 24 36 Kda SubunitMammalian Ste20-Like Protein Kinase 3 N-TerminalMst3/N - Serine/Threonine-Protein Kinase 24 12 Kda SubunitMammalian Ste20-Like Protein Kinase 3 C-TerminalMst3/C |
| Database Links | Reactome: R-HSA-111465Reactome: R-HSA-75153 |
| Cellular Localisation | CytoplasmNucleusMembraneThe Truncated Form (Mst3/N) Translocates To The NucleusColocalizes With Stk38l In The Membrane |
| Alternative Peptide Names | Serine/Threonine-Protein Kinase 24 proteinMammalian Ste20-Like Protein Kinase 3 proteinMst-3 proteinSte20-Like Kinase Mst3 Cleaved Into - Serine/Threonine-Protein Kinase 24 36 Kda Subunit proteinMammalian Ste20-Like Protein Kinase 3 N-Terminal proteinMst3/N - Serine/Threonine-Protein Kinase 24 12 Kda Subunit proteinMammalian Ste20-Like Protein Kinase 3 C-Terminal proteinMst3/C proteinSTK24 proteinMST3 proteinSTK3 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
