| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse VEGFR2-Fc-Tag protein was developed from hek293 and has a target region of Fc-Tag. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 µm filtered PBS solution pH7.4. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was determined by its ability to bind in functional ELISA assay. NA |
| Gene Symbol: |
Kdr |
| Gene ID: |
16542 |
| Uniprot ID: |
VGFR2_MOUSE |
| Immunogen Region: |
ECD |
| Immunogen: |
DNA sequence encoding the extracellular domain of Mouse KDR including a C-terminal human IgG1-Fc tag was expressed in HEK293 Cells. NA |
| Immunogen Sequence: |
ASVGLPGDFL HPPKLSTQKD ILTILANTTL QITCRGQRDL DWLWPNAQRD SEERVLVTEC GGGDSIFCKT LTIPRVVGND TGAYKCSYRD VDIASTVYVY VRDYRSPFIA SVSDQHGIVY ITENKNKTVV IPCRGSISNL NVSLCARYPE KRFVPDGNRI SWDSEIGFTL PSYMISYAGM VFCEAKINDE TYQSIMYIVV VVGYRIYDVI LSPPHEIELS AGEKLVLN |
| Tissue Specificity | Expressed in endothelial cells (at protein level). Detected in embryonic endothelial cells, as well as hematopoietic stem and progenitor cells. Detected in vascular endothelium. Expressed at high levels in adult heart, lung, kidney, brain and skeletal muscle, but is also expressed at lower levels in most other adult tissues. |
| Post Translational Modifications | N-glycosylated. Ubiquitinated. Tyrosine phosphorylation of the receptor promotes its poly-ubiquitination, leading to its degradation via the proteasome or lysosomal proteases. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-949 is important for interaction with SH2D2A/TSAD and VEGFA-mediated reorganization of the actin cytoskeleton. Phosphorylation at Tyr-1173 is important for interaction with PLCG1 and SHB. Phosphorylation at Tyr-1212 is important for interaction with NCK1 and FYN. Dephosphorylated by PTPRJ at Tyr-799, Tyr-949, Tyr-994, Tyr-1052, Tyr-1057, Tyr-1173 and Tyr-1212. The inhibitory disulfide bond between Cys-1022 and Cys-1043 may serve as a specific molecular switch for H(2)S-induced modification that regulates KDR/VEGFR2 function. |
| Function | Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFC and VEGFD. Plays an essential role in the regulation of angiogenesis, vascular development, vascular permeability, and embryonic hematopoiesis. Promotes proliferation, survival, migration and differentiation of endothelial cells. Promotes reorganization of the actin cytoskeleton. Isoforms lacking a transmembrane domain, such as isoform 2, may function as decoy receptors for VEGFA, VEGFC and/or VEGFD. Isoform 2 plays an important role as a negative regulator of VEGFA- and VEGFC-mediated lymphangiogenesis by limiting the amount of free VEGFA and/or VEGFC and by preventing their binding to FLT4. Modulates FLT1 and FLT4 signaling by forming heterodimers. Binding of vascular growth factors to isoform 1 leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, reorganization of the actin cytoskeleton and activation of PTK2/FAK1. Required for VEGFA-mediated induction of NOS2 and NOS3, leading to the production of the signaling molecule nitric oxide (NO) by endothelial cells. Phosphorylates PLCG1. Promotes phosphorylation of FYN, NCK1, NOS3, PIK3R1, PTK2/FAK1 and SRC. |
| Protein Name | Vascular Endothelial Growth Factor Receptor 2Vegfr-2Fetal Liver Kinase 1Flk-1Kinase NykProtein-Tyrosine Kinase Receptor Flk-1Cd Antigen Cd309 |
| Database Links | Reactome: R-MMU-194306Reactome: -MMU-195399Reactome: -MMU-4420097Reactome: -MMU-5218921 |
| Cellular Localisation | Cell JunctionEndoplasmic ReticulumCell MembraneColocalizes With Ern1 And Xbp1 In The Endoplasmic Reticulum In Endothelial Cells In A Vascular Endothelial Growth Factor (Vegf)-Dependent MannerLocalized With Rap1a At Cell-Cell JunctionsIsoform 1: Cell MembraneSingle-Pass Type I Membrane ProteinCytoplasmNucleusCytoplasmic VesicleEarly EndosomeDetected On Caveolae-Enriched Lipid Rafts At The Cell SurfaceIs Recycled From The Plasma Membrane To Endosomes And Back AgainPhosphorylation Triggered By Vegfa Binding Promotes Internalization And Subsequent DegradationVegfa Binding Triggers Internalization And Translocation To The NucleusIsoform 2: Secreted |
| Alternative Protein Names | Vascular Endothelial Growth Factor Receptor 2 proteinVegfr-2 proteinFetal Liver Kinase 1 proteinFlk-1 proteinKinase Nyk proteinProtein-Tyrosine Kinase Receptor Flk-1 proteinCd Antigen Cd309 proteinKdr proteinFlk-1 proteinFlk1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
