Mouse SPTLC2 (Serine Palmitoyltransferase, Long Chain Base Subunit 2) Sandwich ELISA Kit (STJE0012329)
SPECIFICATIONS
General Information
| Short Description | This SPTLC2 Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.055ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | Sptlc2 |
| Gene ID | 20773 |
| Uniprot ID | SPTC2_MOUSE |
| Sample Type | tissue homogenates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in a variety of tissues. Expressed in brains cortices (at protein level). Expressed in brown and white adipose tissues. Expressed in liver. |
| Post Translational Modifications | |
| Function | Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. The SPTLC1-SPTLC2-SPTSSB complex shows a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme displays an ability to use a broader range of acyl-CoAs, without apparent preference. Crucial for adipogenesis. |
| Protein Name | Serine Palmitoyltransferase 2Long Chain Base Biosynthesis Protein 2Lcb 2Long Chain Base Biosynthesis Protein 2aLcb2aSerine-Palmitoyl-Coa Transferase 2Spt 2 |
| Database Links | Reactome: R-MMU-1660661 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane Protein |
| Alternative ELISA Names | Serine Palmitoyltransferase 2 ELISA kitLong Chain Base Biosynthesis Protein 2 ELISA kitLcb 2 ELISA kitLong Chain Base Biosynthesis Protein 2a ELISA kitLcb2a ELISA kitSerine-Palmitoyl-Coa Transferase 2 ELISA kitSpt 2 ELISA kitSptlc2 ELISA kitLcb2 ELISA kit |
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Information sourced from Uniprot.org