Function | Mediates sodium- and chloride-dependent transport of dopamine. Also mediates sodium- and chloride-dependent transport of norepinephrine (also known as noradrenaline). Regulator of light-dependent retinal hyaloid vessel regression, downstream of OPN5 signaling. |
Protein Name | Sodium-Dependent Dopamine TransporterDa TransporterDatSolute Carrier Family 6 Member 3 |
Database Links | Reactome: R-MMU-379401Reactome: -MMU-442660 |
Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionNeuron ProjectionAxonLocalizes To Neurite Tips In Neuronal CellsColocalizes With Septin4 At Axon TerminalsEspecially At The Varicosities |
Alternative ELISA Names | Sodium-Dependent Dopamine Transporter ELISA kitDa Transporter ELISA kitDat ELISA kitSolute Carrier Family 6 Member 3 ELISA kitSlc6a3 ELISA kitDat ELISA kitDat1 ELISA kit |
Specificity | This kit recognizes Mouse DAT in samples. No significant cross-reactivity or interference between Mouse DAT and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
Item | Specifications | Storage |
Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
Stop Solution | 1 vial, 10 mL | 2-8°C |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy | |
Sample Type | Range (%) | Average Recovery (%) |
Serum(n=8) | 93-107 | 98 |
EDTA plasma (n=8) | 84-97 | 91 |
Cell culture media (n=8) | 85-99 | 91 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
Mean (pg/mL) | 22.94 | 71.32 | 212.65 | 23.07 | 72.20 | 200.28 |
Standard deviation | 1.22 | 3.16 | 9.25 | 1.53 | 3.42 | 10.59 |
CV (%) | 5.32 | 4.43 | 4.35 | 6.63 | 4.74 | 5.29 |