Mouse RUNX2 protein (Recombinant) (N-His) (STJP014885)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenMus musculus (Mouse)
General Information
| Short Description | Recombinant-Mouse RUNX2-N-His protein was developed from e.coli and has a target region of N-His. For use in research applications. |
| Applications | ELISA/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for this information. |
Target Information
| Gene Symbol | Runx2 |
| Gene ID | 12393 |
| Uniprot ID | RUNX2_MOUSE |
| Immunogen | Mus musculus (Mouse) |
| Immunogen Region | Thr187-Arg315 |
Additional Info
| Post Translational Modifications | Phosphorylated.probably by MAP kinases (MAPK). Phosphorylation by HIPK3 is required for the SPEN/MINT and FGF2 transactivation during osteoblastic differentiation. Phosphorylation at Ser-537 by CDK1 promotes endothelial cell proliferation required for tumor angiogenesis probably by facilitating cell cycle progression. |
| Function | Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters. Inhibits KAT6B-dependent transcriptional activation. In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). |
| Protein Name | Runt-Related Transcription Factor 2Acute Myeloid Leukemia 3 ProteinCore-Binding Factor Subunit Alpha-1Cbf-Alpha-1Oncogene Aml-3Osteoblast-Specific Transcription Factor 2Osf-2Polyomavirus Enhancer-Binding Protein 2 Alpha A SubunitPea2-Alpha APebp2-Alpha ASl3-3 Enhancer Factor 1 Alpha A SubunitSl3/Akv Core-Binding Factor Alpha A Subunit |
| Database Links | Reactome: R-MMU-8878166 Q08775-5Reactome: -MMU-8939902 Q08775-5Reactome: -MMU-8940973 Q08775-5Reactome: -MMU-8941326 Q08775-5 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Protein Names | Runt-Related Transcription Factor 2 proteinAcute Myeloid Leukemia 3 Protein proteinCore-Binding Factor Subunit Alpha-1 proteinCbf-Alpha-1 proteinOncogene Aml-3 proteinOsteoblast-Specific Transcription Factor 2 proteinOsf-2 proteinPolyomavirus Enhancer-Binding Protein 2 Alpha A Subunit proteinPea2-Alpha A proteinPebp2-Alpha A proteinSl3-3 Enhancer Factor 1 Alpha A Subunit proteinSl3/Akv Core-Binding Factor Alpha A Subunit proteinRunx2 proteinAml3 proteinCbfa1 proteinOsf2 proteinPebp2a protein |
Information sourced from Uniprot.org