| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Mouse R-Spondin1-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability recombinant mouse RSPO1 to induce activation of beta catenin in Luciferase assay in the presence of recombinant mouse Wnt3a, the calulated ED50 is 50-200 ng/ml. NA |
| Gene Symbol: |
Rspo1 |
| Gene ID: |
192199 |
| Uniprot ID: |
RSPO1_MOUSE |
| Immunogen Region: |
SER21-GLN265 |
| Immunogen: |
Optimized DNA sequence encoding peptidase domain of Mouse R-Spondin1 (SER21-GLN265 ) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed in the dorsal part of the neural tube on 10 and 12 dpc, especially in the boundary region between roof plate and neuroepithelium. This expression is enhanced in the rostral part. Also expressed in other tissues such as truncal region neighboring forelimbs and mesenchymal tissues around the nasal cavity. |
| Function | Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Has a essential roles in ovary determination. Regulates Wnt signaling by antagonizing DKK1/KREM1-mediated internalization of LRP6 through an interaction with KREM1. |
| Protein Name | R-Spondin-1Cysteine-Rich And Single Thrombospondin Domain-Containing Protein 3Cristin-3Mcristin-3Roof Plate-Specific Spondin-1 |
| Database Links | Reactome: R-MMU-4641263 |
| Cellular Localisation | SecretedNucleusSeems To Mainly Localize To Nucleoli |
| Alternative Protein Names | R-Spondin-1 proteinCysteine-Rich And Single Thrombospondin Domain-Containing Protein 3 proteinCristin-3 proteinMcristin-3 proteinRoof Plate-Specific Spondin-1 proteinRspo1 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
