• Mouse Nos3 (Nitric oxide synthase, endothelial) Sandwich ELISA (STJE0004531)

Mouse Nos3 (Nitric oxide synthase, endothelial) Sandwich ELISA (STJE0004531)

SKU:
STJE0004531

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Applications: ELISA
Reactivity: Mouse
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This Nos3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: Nos3
Gene ID: 18127
Uniprot ID: NOS3_MOUSE
Specificity: This kit recognizes Mouse NOS3/eNOS in samples. No significant cross-reactivity or interference between Mouse NOS3/eNOS and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. May play a significant role in normal and abnormal limb development.
Protein Name Nitric Oxide Synthase 3
Constitutive Nos
Cnos
Ec-Nos
Nos Type Iii
Nosiii
Nitric Oxide Synthase - Endothelial
Endothelial Nos
Enos
Database Links Reactome: R-MMU-1222556
Reactome: -MMU-1474151
Reactome: -MMU-203615
Reactome: -MMU-203641
Reactome: -MMU-203754
Reactome: -MMU-392154
Reactome: -MMU-5218920
Reactome: -MMU-9009391
Cellular Localisation Membrane
Caveola
Cytoplasm
Cytoskeleton
Golgi Apparatus
Cell Membrane
Specifically Associates With Actin Cytoskeleton In The G2 Phase Of The Cell Cycle
Which Is Favored By Interaction With Nosip And Results In A Reduced Enzymatic Activity
Alternative ELISA Names Nitric Oxide Synthase 3 ELISA kit
Constitutive Nos ELISA kit
Cnos ELISA kit
Ec-Nos ELISA kit
Nos Type Iii ELISA kit
Nosiii ELISA kit
Nitric Oxide Synthase - Endothelial ELISA kit
Endothelial Nos ELISA kit
Enos ELISA kit
Nos3 ELISA kit
Ecnos ELISA kit
Specificity This kit recognizes Mouse NOS3/eNOS in samples. No significant cross-reactivity or interference between Mouse NOS3/eNOS and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma (n=8) 90-104 97
Cell culture media (n=8) 92-104 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 45.50 109.20 426.30 44.30 119.40 394.80
Standard deviation 3.20 5.10 17.90 2.40 6.20 18.60
CV (%) 7.03 4.67 4.20 5.42 5.19 4.71
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance