This Nos2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications
ELISA
Reactivity
Mouse
Sensitivity
0.19ng/mL
Detection Limit
0.31~20ng/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
Polyubiquitinated.mediated by SPSB1, SPSB2 and SPSB4, leading to proteasomal degradation.
Function
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such PTGS2/COX2. As component of the iNOS-S100A8/9 transnitrosylase complex involved in the selective inflammatory stimulus-dependent S-nitrosylation of GAPDH implicated in regulation of the GAIT complex activity and probably multiple targets including ANXA5, EZR, MSN and VIM. Involved in inflammation, enhances the synthesis of pro-inflammatory mediators such as IL6 and IL8.
Protein Name
Nitric Oxide Synthase - InducibleInducible No SynthaseInducible NosInosMacrophage NosMac-NosNos Type IiPeptidyl-Cysteine S-Nitrosylase Nos2
CytoplasmCytosolLocalizes As Discrete Foci Scattered Throughout The Cytosol And In The Presence Of Spsb1 And Spsb4Exhibits A More Diffuse Cytosolic Localization
Alternative ELISA Names
Nitric Oxide Synthase - Inducible ELISA kitInducible No Synthase ELISA kitInducible Nos ELISA kitInos ELISA kitMacrophage Nos ELISA kitMac-Nos ELISA kitNos Type Ii ELISA kitPeptidyl-Cysteine S-Nitrosylase Nos2 ELISA kitNos2 ELISA kitInosl ELISA kit
Specificity
This kit recognizes Mouse NOS2/iNOS in samples. No significant cross-reactivity or interference between Mouse NOS2/iNOS and analogues was observed.
