Mouse NNMT (Nicotinamide-N-Methyltransferase) Sandwich ELISA Kit Ready-To-Use Kit (STJE0017383)
SPECIFICATIONS
General Information
| Short Description | This NNMT Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Mouse |
| Sensitivity | 0.055ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 3 hrs |
Target Information
| Gene Symbol | Nnmt |
| Gene ID | 18113 |
| Uniprot ID | NNMT_MOUSE |
| Immunogen Region | Ready-To-Use |
| Sample Type | serum, plasma or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in white adipose tissue and liver (at protein level). |
| Post Translational Modifications | Deiminated by PADI1 and PADI2. |
| Function | Catalyzes the N-methylation of nicotinamide using the universal methyl donor S-adenosyl-L-methionine to form N1-methylnicotinamide and S-adenosyl-L-homocysteine, a predominant nicotinamide/vitamin B3 clearance pathway. Plays a central role in regulating cellular methylation potential, by consuming S-adenosyl-L-methionine and limiting its availability for other methyltransferases. Actively mediates genome-wide epigenetic and transcriptional changes through hypomethylation of repressive chromatin marks, such as H3K27me3. In a developmental context, contributes to low levels of the repressive histone marks that characterize pluripotent embryonic stem cell pre-implantation state. Acts as a metabolic regulator primarily on white adipose tissue energy expenditure as well as hepatic gluconeogenesis and cholesterol biosynthesis. In white adipocytes, regulates polyamine flux by consuming S-adenosyl-L-methionine which provides for propylamine group in polyamine biosynthesis, whereas by consuming nicotinamide controls NAD(+) levels through the salvage pathway. Via its product N1-methylnicotinamide regulates protein acetylation in hepatocytes, by repressing the ubiquitination and increasing the stability of SIRT1 deacetylase. Can also N-methylate other pyridines structurally related to nicotinamide and play a role in xenobiotic detoxification. |
| Protein Name | Nicotinamide N-Methyltransferase |
| Database Links | Reactome: R-MMU-156581Reactome: -MMU-197264 |
| Cellular Localisation | Cytoplasm |
| Alternative ELISA Names | Nicotinamide N-Methyltransferase ELISA kitNnmt ELISA kit |
| output |
Information sourced from Uniprot.org