| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 9.65 pg/mL |
| Detection Limit: | 31.25-2000 pg/mL |
| Short Description: | This NGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Ngf |
| Gene ID: | 18049 |
| Uniprot ID: | NGF_MOUSE |
| Specificity: | This kit recognizes Mouse NGF in samples. No significant cross-reactivity or interference between Mouse NGF and analogues was observed. |
| Sample Type: | serum, plasma and other biological fluids |
| Tissue Specificity | Detected in submaxillary gland (at protein level). Highly expressed in male submaxillary gland. Levels are much lower in female submaxillary gland. |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival. The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. |
| Protein Name | Beta-Nerve Growth Factor Beta-Ngf |
| Database Links | Reactome: R-MMU-167060 Reactome: -MMU-170968 Reactome: -MMU-170984 Reactome: -MMU-177504 Reactome: -MMU-187042 Reactome: -MMU-198203 Reactome: -MMU-205017 Reactome: -MMU-205025 Reactome: -MMU-205043 Reactome: -MMU-209543 Reactome: -MMU-209560 Reactome: -MMU-209563 |
| Cellular Localisation | Secreted Endosome Lumen Prongf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr |
| Alternative ELISA Names | Beta-Nerve Growth Factor ELISA kit Beta-Ngf ELISA kit Ngf ELISA kit Ngfb ELISA kit |
| Specificity | This kit recognizes Mouse NGF in samples. No significant cross-reactivity or interference between Mouse NGF and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-106 | 98 |
| EDTA plasma (n=8) | 85-100 | 92 |
| Cell culture media (n=8) | 93-106 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 92.81 | 252.60 | 909.20 | 94.33 | 261.26 | 973.95 |
| Standard deviation | 6.04 | 14.75 | 36.19 | 6.09 | 14.89 | 35.55 |
| CV (%) | 6.51 | 5.84 | 3.98 | 6.46 | 5.70 | 3.65 |
