Mouse Malate dehydrogenase 2/MDH2 protein (Recombinant) (His-Tag) (STJP019364)

SPECIFICATIONS
HostE.coli
ImmunogenMouse
STJP019364
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General Information

Short DescriptionRecombinant-Mouse Malate dehydrogenase 2/MDH2-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE/Enzyme Activity
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration1 mg/mL
FormulationLiquid in phosphate-Buffered Saline (pH 7.4) containing 20% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
ImmunoreactivitySpecific activity is > 800unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxaloacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C

Target Information

Gene SymbolMdh2
Gene ID17448
Uniprot IDMDHM_MOUSE
Accession NumberNP_032643
ImmunogenMouse
Immunogen Region25-338aa
Immunogen SequenceMGSSHHHHHH SSGLVPRGSH MAKVAVLGAS GGIGQPLSLL LKNSPLVSRL TLYDIAHTPG VAADLSHIET RANVKGYLGP EQLPDCLKGC DVVVIPAGVP RKPGMTRDDL FNTNATIVAT LTAACAQHCP EAMVCIIANP VNSTIPITAE VFKKHGVYNP NKIFGVTTLD IVRANTFVAE LKGLDPARVN VPVIGGHAGK TIIPLISQCT PKVDFPQDQL ATLTGRIQ

Additional Info

Post Translational Modifications Acetylation is enhanced after treatment either with trichostin A (TCA) or with nicotinamide (NAM) with the appearance of tri- and tetraacetylations. Glucose also increases acetylation. Acetylation of Lys-239 and Lys-314 is observed in liver mitochondria from fasted mice but not from fed mice.
Protein Name Malate Dehydrogenase - Mitochondrial
Database Links Reactome: R-MMU-71403
Reactome: -MMU-9837999
Reactome: -MMU-9856872
Cellular Localisation Mitochondrion Matrix
Alternative Protein Names Malate Dehydrogenase - Mitochondrial protein
Mdh2 protein
Mor1 protein

Information sourced from Uniprot.org

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