| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: |
0.103ng/mL |
| Detection Limit: |
0.312-20ng/mL |
| Short Description: |
This LXRa Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates or other biological fluids.. |
| Storage Instruction: |
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: |
3 hrs |
| Gene Symbol: |
Nr1h3 |
| Gene ID: |
22259 |
| Uniprot ID: |
NR1H3_MOUSE |
| Immunogen Region: |
Ready-To-Use |
| Sample Type: |
tissue homogenates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Ubiquitinated. Ubiquitination by UBR5 leads to its degradation: UBR5 specifically recognizes and binds ligand-bound NR1H3 when it is not associated with coactivators (NCOAs). In presence of NCOAs, the UBR5-degron is not accessible, preventing its ubiquitination and degradation. |
| Function | Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Interaction with retinoic acid receptor (RXR) shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. LXRES are DR4-type response elements characterized by direct repeats of two similar hexanuclotide half-sites spaced by four nucleotides. Plays an important role in the regulation of cholesterol homeostasis, regulating cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis. Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. |
| Protein Name | Oxysterols Receptor Lxr-AlphaLiver X Receptor AlphaNuclear Receptor Subfamily 1 Group H Member 3 |
| Database Links | Reactome: R-MMU-383280Reactome: -MMU-4090294Reactome: -MMU-8866427Reactome: -MMU-9029569Reactome: -MMU-9623433 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative ELISA Names | Oxysterols Receptor Lxr-Alpha ELISA kitLiver X Receptor Alpha ELISA kitNuclear Receptor Subfamily 1 Group H Member 3 ELISA kitNr1h3 ELISA kitLxra ELISA kit |
| output | |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
