Mouse Lptn/XCL1 (Lymphotactin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This mouse Lptn/XCL1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Mouse |
| Sensitivity | 9.38pg/mL |
| Detection Limit | 15.63~1000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | Xcl1 |
| Gene ID | 16963 |
| Uniprot ID | XCL1_MOUSE |
| Specificity | This kit recognizes Mouse Lptn/XCL1 in samples. No significant cross-reactivity or interference between Mouse Lptn/XCL1 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expressed in activated CD8(+) T cells. In the thymus, expressed by medullary thymic epithelial cells. |
| Function | Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment. |
| Protein Name | LymphotactinC Motif Chemokine 1Cytokine Scm-1LymphotaxinSmall-Inducible Cytokine C1 |
| Database Links | Reactome: R-MMU-380108Reactome: -MMU-416476 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Lymphotactin CLIA kitC Motif Chemokine 1 CLIA kitCytokine Scm-1 CLIA kitLymphotaxin CLIA kitSmall-Inducible Cytokine C1 CLIA kitXcl1 CLIA kitLptn CLIA kitLtn CLIA kitScyc1 CLIA kit |
| Specificity | This kit recognizes Mouse Lptn/XCL1 in samples. No significant cross-reactivity or interference between Mouse Lptn/XCL1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-103 | 95 |
| EDTA plasma(n=8) | 88-103 | 95 |
| Cell culture media(n=8) | 89-102 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 50.45 | 137.81 | 354.18 | 47.46 | 129.87 | 348.52 |
| Standard deviation | 5.40 | 11.16 | 27.84 | 4.47 | 11.87 | 26.77 |
| CV (%) | 10.70 | 8.10 | 7.86 | 9.42 | 9.14 | 7.68 |