Mouse Leukemia inhibitory factor/LIF protein (Recombinant) (C-His&Avi) (STJP001407)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Mouse Leukemia inhibitory factor/LIF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser24-Phe203) of mouse LIF (Accession #NP_032527.1) fused with a 6×His, Avi tag at the C-terminus.
General Information
| Short Description | Recombinant-Mouse Leukemia inhibitory factor/LIF-C-His&Avi protein was developed in hek293 cells using the region Ser24-Phe203. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 35.675-142.7 ng/mL, corresponding to a specific activity of 7.00×104~2.80×104 units/mg. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | Lif |
| Gene ID | 16878 |
| Uniprot ID | LIF_MOUSE |
| Immunogen | Recombinant Mouse Leukemia inhibitory factor/LIF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser24-Phe203) of mouse LIF (Accession #NP_032527.1) fused with a 6×His, Avi tag at the C-terminus. |
| Immunogen Region | Ser24-Phe203 |
| Immunogen Sequence | SPLPITPVNATCAIRHPCHG NLMNQIKNQLAQLNGSANAL FISYYTAQGEPFPNNVEKLC APNMTDFPSFHGNGTEKTKL VELYRMVAYLSASLTNITRD QKVLNPTAVSLQVKLNATID VMRGLLSNVLCRLCNKYRVG HVDVPPVPDHSDKEAFQRKK LGCQLLGTYKQVISVVVQAF |
Additional Info
| Protein Name | Leukemia Inhibitory FactorLifDifferentiation-Stimulating FactorD Factor |
| Database Links | Reactome: R-MMU-6788467 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Leukemia Inhibitory Factor proteinLif proteinDifferentiation-Stimulating Factor proteinD Factor proteinLif protein |
Information sourced from Uniprot.org