| Short Description : | Recombinant-Mouse Leukemia inhibitory factor/LIF-C-His&Avi protein was developed in hek293 cells using the region Ser24-Phe203. For use in research applications. |
| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Conjugation: | Unconjugated |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity: | Measured in a cell proliferation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is 35.675-142.7 ng/mL, corresponding to a specific activity of 7.00×104~2.80×104 units/mg. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Gene Symbol: | Lif |
| Gene ID: | 16878 |
| Uniprot ID: | LIF_MOUSE |
| Immunogen: | Recombinant Mouse Leukemia inhibitory factor/LIF Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser24-Phe203) of mouse LIF (Accession #NP_032527.1) fused with a 6×His, Avi tag at the C-terminus. |
| Immunogen Region: | Ser24-Phe203 |
| Immunogen Sequence: | SPLPITPVNATCAIRHPCHG NLMNQIKNQLAQLNGSANAL FISYYTAQGEPFPNNVEKLC APNMTDFPSFHGNGTEKTKL VELYRMVAYLSASLTNITRD QKVLNPTAVSLQVKLNATID VMRGLLSNVLCRLCNKYRVG HVDVPPVPDHSDKEAFQRKK LGCQLLGTYKQVISVVVQAF |
| Protein Name | Leukemia Inhibitory FactorLifDifferentiation-Stimulating FactorD Factor |
| Database Links | Reactome: R-MMU-6788467 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Leukemia Inhibitory Factor proteinLif proteinDifferentiation-Stimulating Factor proteinD Factor proteinLif protein |
Information sourced from Uniprot.org
