| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 23.44pg/mL |
| Detection Limit: | 39.06~2500pg/mL |
| Short Description: | This Lcn2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Lcn2 |
| Gene ID: | 16819 |
| Uniprot ID: | NGAL_MOUSE |
| Specificity: | This kit recognizes Mouse NGAL in samples. No significant cross-reactivity or interference between Mouse NGAL and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. |
| Function | Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,3-dihydroxybenzoic acid (2,3-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity.limits bacterial proliferation by sequestering iron bound to microbial siderophores, such as enterobactin. Can also bind siderophores from M.tuberculosis. |
| Protein Name | Neutrophil Gelatinase-Associated Lipocalin Ngal Lipocalin-2 Oncogene 24p3 24p3 Sv-40-Induced 24p3 Protein Siderocalin Lcn2 P25 |
| Database Links | Reactome: R-MMU-6798695 Reactome: -MMU-6799990 Reactome: -MMU-917937 |
| Cellular Localisation | Secreted Cytoplasmic Granule Lumen Cytoplasmic Vesicle Lumen Upon Binding To The Slc22a17 (24p3r) Receptor It Is Internalized Releases The Bound Iron In The Acidic Lumen Of Cytoplasmic Vesicles |
| Alternative ELISA Names | Neutrophil Gelatinase-Associated Lipocalin ELISA kit Ngal ELISA kit Lipocalin-2 ELISA kit Oncogene 24p3 ELISA kit 24p3 ELISA kit Sv-40-Induced 24p3 Protein ELISA kit Siderocalin Lcn2 ELISA kit P25 ELISA kit Lcn2 ELISA kit |
| Specificity | This kit recognizes Mouse NGAL in samples. No significant cross-reactivity or interference between Mouse NGAL and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-105 | 97 |
| EDTA plasma (n=8) | 89-102 | 95 |
| Cell culture media (n=8) | 91-108 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 119.40 | 390.07 | 1093.66 | 121.64 | 352.20 | 1131.18 |
| Standard deviation | 6.93 | 17.28 | 43.64 | 7.99 | 18.81 | 60.41 |
| CV (%) | 5.80 | 4.43 | 3.99 | 6.57 | 5.34 | 5.34 |
