Mouse LC3B/MAP1LC3B protein (Recombinant) (His-Tag) (STJP019347)
SPECIFICATIONS
HostE.coli
ImmunogenMouse
General Information
| Short Description | Recombinant-Mouse LC3B/MAP1LC3B-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM MES buffer (pH 6.0) containing 0.1M NaCl, 1mM DTT, 30% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | Map1lc3b |
| Gene ID | 67443 |
| Uniprot ID | MLP3B_MOUSE |
| Accession Number | NP_080436 |
| Immunogen | Mouse |
| Immunogen Region | 1-120aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSHMPSEKT FKQRRSFEQR VEDVRLIREQ HPTKIPVIIE RYKGEKQLPV LDKTKFLVPD HVNMSELIKI IRRRLQLNAN QAFFLLVNGH SMVSVSTPIS EVYESERDED GFLYMVYASQ ETFG |
Additional Info
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. ATG4B constitutes the major protein for proteolytic activation. ATG4D is the main enzyme for delipidation activity. Phosphorylation by PKA inhibits conjugation of phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity. Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. |
| Function | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover. Upon nutrient stress, directly recruits cofactor JMY to the phagophore membrane surfaces and promotes JMY's actin nucleation activity and autophagosome biogenesis during autophagy. |
| Protein Name | Microtubule-Associated Protein 1 Light Chain 3 BetaAutophagy-Related Protein Lc3 BAutophagy-Related Ubiquitin-Like Modifier Lc3 BMap1 Light Chain 3-Like Protein 2Microtubule-Associated Proteins 1a/1b Light Chain 3bMap1a/Map1b Lc3 BMap1a/Map1b Light Chain 3 B |
| Database Links | Reactome: R-MMU-1632852Reactome: -MMU-5205685Reactome: -MMU-8854214Reactome: -MMU-8934903Reactome: -MMU-9664873Reactome: -MMU-9755511 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneLipid-AnchorEndomembrane SystemMitochondrion MembraneCytoplasmCytoskeletonLc3-Ii Binds To The Autophagic MembranesLc3-Ii Localizes With The Mitochondrial Inner Membrane During Parkin-Mediated MitophagyAlso Localizes To Discrete Punctae Along The Ciliary Axoneme |
| Alternative Protein Names | Microtubule-Associated Protein 1 Light Chain 3 Beta proteinAutophagy-Related Protein Lc3 B proteinAutophagy-Related Ubiquitin-Like Modifier Lc3 B proteinMap1 Light Chain 3-Like Protein 2 proteinMicrotubule-Associated Proteins 1a/1b Light Chain 3b proteinMap1a/Map1b Lc3 B proteinMap1a/Map1b Light Chain 3 B proteinMap1lc3b proteinMap1alc3 proteinMap1lc3 protein |
Information sourced from Uniprot.org