| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 18.75pg/mL |
| Detection Limit: | 31.25-2000pg/mL |
| Short Description: | This KIM-1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Havcr1 |
| Gene ID: | 171283 |
| Uniprot ID: | HAVR1_MOUSE |
| Specificity: | This kit recognizes Mouse KIM-1 in samples. No significant cross-reactivity or interference between Mouse KIM-1 and analogues was observed. |
| Sample Type: | Serum, plasma and other biological fluids |
| Function | Phosphatidylserine receptor that plays an important functional role in regulatory B-cells homeostasis including generation, expansion and suppressor functions. As P-selectin/SELPLG ligand, plays a specialized role in activated but not naive T-cell trafficking during inflammatory responses. Controls thereby T-cell accumulation in the inflamed central nervous system (CNS) and the induction of autoimmune disease. Regulates also expression of various anti-inflammatory cytokines and co-inhibitory ligands including IL10. Acts as a regulator of T-cell proliferation. May play a role in kidney injury and repair. |
| Protein Name | Hepatitis A Virus Cellular Receptor 1 Homolog Havcr-1 Kidney Injury Molecule 1 Kim-1 T Cell Immunoglobulin And Mucin Domain-Containing Protein 1 Timd-1 T Cell Membrane Protein 1 T-Cell Immunoglobulin Mucin Receptor 1 Tim-1 Cd Antigen Cd365 |
| Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein |
| Alternative ELISA Names | Hepatitis A Virus Cellular Receptor 1 Homolog ELISA kit Havcr-1 ELISA kit Kidney Injury Molecule 1 ELISA kit Kim-1 ELISA kit T Cell Immunoglobulin And Mucin Domain-Containing Protein 1 ELISA kit Timd-1 ELISA kit T Cell Membrane Protein 1 ELISA kit T-Cell Immunoglobulin Mucin Receptor 1 ELISA kit Tim-1 ELISA kit Cd Antigen Cd365 ELISA kit Havcr1 ELISA kit Kim1 ELISA kit Tim1 ELISA kit Timd1 ELISA kit |
| Specificity | This kit recognizes Mouse KIM-1 in samples. No significant cross-reactivity or interference between Mouse KIM-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (50×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-107 | 99 |
| EDTA plasma (n=8) | 94-108 | 100 |
| Cell culture media (n=8) | 92-105 | 99 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 98.77 | 219.54 | 982.15 | 105.73 | 220.70 | 904.18 |
| Standard deviation | 6.36 | 10.08 | 48.22 | 6.40 | 12.01 | 45.66 |
| CV (%) | 6.44 | 4.59 | 4.91 | 6.05 | 5.44 | 5.05 |
